Abstract
Caecal samples from 350 Danish bacon pigs were investigated for salmonella using three methods of isolation. (1) Direct inoculation of 1 g of faeces into 10 ml of Muller-Kaufmann medium (MK medium) with addition of 0.3% Teepol 610 and subculture on Brilliant Green lactose sucrose phenol-red agar (BLSF agar) with 0.3% Teepol 610. (2) Pre-enrichment of 5 g of faeces into buffered peptone water with addition of 1% Teepol 610 followed by enrichment of 1 ml in 10 ml MK medium with 1% Teepol 610 and subculture on BLSF agar with 0.3% Teepol. (3) Incubation of 0.1 ml of the pre-enrichment (2) into 10 ml Rappaport-Vassiliadis medium (RV 10 medium) incubated at 43 degrees C, subculture on BLSF agar. The MK media with and without pre-enrichment yielded higher findings than the RV 10 media. In total, 28 (8%) of the pigs were found positive, representing 11 (7.4%) of a total of 142 herds investigated. Lymph glands were collected at a later date from six of the positive herds. Five of the herds were found positive. The number of salmonellas in the glands was low, probably less than ten per gram.
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- EMBERLEY N. W., JAMESON J. E. A substitute for bile salts in culture media. J Gen Microbiol. 1956 Aug;15(1):198–204. doi: 10.1099/00221287-15-1-198. [DOI] [PubMed] [Google Scholar]
- Haddock R. L. Efficacy of examining rectal swabs to detect swine Salmonella carriers. Am J Vet Res. 1970 Aug;31(8):1509–1512. [PubMed] [Google Scholar]
- Harvey R. W., Price T. H. A comparison of two modifications of Rappaport's enrichment medium (R25 and RV) for the isolation of salmonellas from sewage polluted natural water. J Hyg (Lond) 1983 Dec;91(3):451–458. doi: 10.1017/s0022172400060496. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Harvey R. W., Price T. H., Xirouchaki E. Comparison of selenite F, Muller-Kauffmann tetrathionate and Rappaport's medium for the isolation of salmonellas from sewage-polluted natural water using a pre-enrichment technique. J Hyg (Lond) 1979 Dec;83(3):451–460. doi: 10.1017/s0022172400026292. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KONFORTI N., NAVON B., RAPPAPORT F. A new enrichment medium for certain Salmonellae. J Clin Pathol. 1956 Aug;9(3):261–266. doi: 10.1136/jcp.9.3.261. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Köhler W., Ghatak S. N., Rische H., Ziesché K. Arylsulfatase-Bildung durch Mikroorganismen, besonders durch Salmonella paratyphi B. Beziehungen zwischen Lysotyp und Enzymbildung. Zentralbl Bakteriol Orig. 1966 Dec;201(4):482–488. [PubMed] [Google Scholar]
- Skovgaard N., Nielsen B. B. Salmonella in pigs and animal feeding stuffs in England and Wales and in Denmark. J Hyg (Lond) 1972 Mar;70(1):127–140. doi: 10.1017/s0022172400022178. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Vassiliadis P. The Rappaport-Vassiliadis (RV) enrichment medium for the isolation of salmonellas: an overview. J Appl Bacteriol. 1983 Feb;54(1):69–76. doi: 10.1111/j.1365-2672.1983.tb01302.x. [DOI] [PubMed] [Google Scholar]
- Vassiliadis P., Trichopoulos D., Kalapothaki V., Sérié C. Isolation of Salmonella with the use of 100 ml of the R10 modification of Rappaport's enrichment medium. J Hyg (Lond) 1981 Aug;87(1):35–41. doi: 10.1017/s0022172400069217. [DOI] [PMC free article] [PubMed] [Google Scholar]
- van Schothorst M., Renaud A. M. Dynamics of salmonella isolation with modified Rappaport's medium (R10). J Appl Bacteriol. 1983 Apr;54(2):209–215. doi: 10.1111/j.1365-2672.1983.tb02609.x. [DOI] [PubMed] [Google Scholar]