Abstract
Three of four extreme methods of immunization completely failed to protect mice against challenge with the homologous strain of Fusobacterium necrophorum. Unsuccessful vaccines included (1) broth culture killed by mild heat and emulsified with Freund's complete adjuvant, and (2) a homogenate of heavily infected mouse brains, inactivated by mild heat and given in two doses. Also unsuccessful as a method of immunization was the production of a severe subcutaneous infection with F. necrophorum, followed by curative treatment with metronidazole. Slight but significant protection against subcutaneous challenge resulted, however, from two such infections given in rapid succession. It would appear that the main virulence factors of F. necrophorum are only weakly immunogenic, and the experiments give little encouragement to the prospect of an effective necrobacillosis vaccine.
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- Abe P. M., Holland J. W., Stauffer L. R. Immunization of mice against Fusobacterium necrophorum infection by perenteral or oral administration of vaccine. Am J Vet Res. 1978 Jan;39(1):115–118. [PubMed] [Google Scholar]
- Cameron C. M., Fuls W. J. Failure to induce in rabbits effective immunity to a mixed infection of Fusobacterium necrophorum and Corynebacterium pyogenes with a combined bacterin. Onderstepoort J Vet Res. 1977 Dec;44(4):253–255. [PubMed] [Google Scholar]
- Deacon A. G., Duerden B. I., Holbrook W. P. Gas-lipuid chromatographic analysis of metabolic products in the identification of bacteroidaceae of clinical interest. J Med Microbiol. 1978 May;11(2):81–99. doi: 10.1099/00222615-11-2-81. [DOI] [PubMed] [Google Scholar]
- EVANS D. G., PERKINS F. T. An improved method for testing the ability of pertussis vaccines to produce agglutinin. J Pathol Bacteriol. 1954 Jul;68(1):251–257. doi: 10.1002/path.1700680131. [DOI] [PubMed] [Google Scholar]
- Egerton J. R., Roberts D. S. Vaccination against ovine foot-rot. J Comp Pathol. 1971 Apr;81(2):179–185. doi: 10.1016/0021-9975(71)90091-0. [DOI] [PubMed] [Google Scholar]
- Garcia M. M., Dorward W. J., Alexander D. C., Magwood S. E., McKay K. A. Results of a preliminary trial with Sphaerophorus necrophorus toxoids to control liver abscesses in feedlot cattle. Can J Comp Med. 1974 Jul;38(3):222–226. [PMC free article] [PubMed] [Google Scholar]
- Hooker J. M., Smith G. R., Milligan R. A. Differentiation of Mycoplasma mycoides subsp. mycoides from certain closely related caprine mycoplasmas by mycoplasmaemia and cross-protection tests in mice. J Hyg (Lond) 1979 Jun;82(3):407–418. doi: 10.1017/s0022172400053936. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Katitch R. V. Les problèmes de l'étiologie et de l'immunoprophylaxie dans le piétin du mouton. Comp Immunol Microbiol Infect Dis. 1979;2(1):55–59. doi: 10.1016/0147-9571(79)90058-4. [DOI] [PubMed] [Google Scholar]
- Oliphant J. C., Parsons R., Smith G. R. Aetiological agents of necrobacillosis in captive wallabies. Res Vet Sci. 1984 May;36(3):382–384. [PubMed] [Google Scholar]
- Smith G. R. Factors affecting bacteriaemia in mice inoculated with Mycoplasma mycoides var. mycoides. J Comp Pathol. 1968 Jul;78(3):267–274. doi: 10.1016/0021-9975(68)90002-9. [DOI] [PubMed] [Google Scholar]
- Smith G. R., Oliphant J. C., Parsons R. The pathogenic properties of Fusobacterium and Bacteroides species from wallabies and other sources. J Hyg (Lond) 1984 Apr;92(2):165–175. doi: 10.1017/s0022172400064184. [DOI] [PMC free article] [PubMed] [Google Scholar]