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. 1985 Oct;95(2):337–344. doi: 10.1017/s0022172400062756

An evaluation of commercially available dehydrated Rappaport-Vassiliadis medium for the isolation of salmonellae from poultry.

C R Fricker, E Quail, L McGibbon, R W Girdwood
PMCID: PMC2129555  PMID: 4067294

Abstract

A total of 745 samples of chicken giblets was cultured to determine the relative efficiency of a commercially available Rappaport-Vassiliadis medium (RV-Oxoid). Experiments to determine the optimum inoculation ratio showed that 1:100 was superior to the other ratios tested. Comparison of RV-Oxoid with standard RV and RV-medium prepared using soya peptone (RV-soya) showed that after 24 h RV-soya was significantly better than RV-Oxoid (P less than 0.05), although there was no significant difference between standard RV and RV-Oxoid. Furthermore, when the duration of incubation was extended to 48 h there was no significant difference between the three media (P greater than 0.25). We conclude that RV-Oxoid is a satisfactory product for the isolation of salmonellae from poultry, providing that it is inoculated at a ratio of 1:100 and is incubated for 48 h. Its use can therefore be recommended to laboratories who wish to use a dehydrated medium.

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Selected References

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