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. 1986 Aug;167(2):744–748. doi: 10.1128/jb.167.2.744-748.1986

Genetic analysis of Myxococcus xanthus and isolation of gene replacements after transduction under conditions of limited homology.

K A O'Connor, D R Zusman
PMCID: PMC212957  PMID: 3090023

Abstract

Genetic analysis of Myxococcus xanthus is greatly facilitated by the ability to introduce cloned DNA into M. xanthus to generate gene replacement and merodiploid strains. However, gene replacement strains are difficult to obtain when the region(s) of homology between the cloned DNA and the M. xanthus chromosome is limited (less than 1 kilobase). We found that gene replacements can be obtained at an increased frequency by a two-step procedure involving the use of bacteriophage P1 to isolate merodiploid strains followed by generalized transduction to another M. xanthus strain by using phage Mx4.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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