Skip to main content
The Journal of Hygiene logoLink to The Journal of Hygiene
. 1986 Apr;96(2):277–289. doi: 10.1017/s0022172400066055

Institutional outbreaks of rotavirus diarrhoea: potential role of fomites and environmental surfaces as vehicles for virus transmission.

S A Sattar, N Lloyd-Evans, V S Springthorpe, R C Nair
PMCID: PMC2129638  PMID: 3701042

Abstract

To assess the potential of fomites and environmental surfaces as vehicles in the transmission of rotaviral diarrhoea, disks (1 cm diameter) of various porous and non-porous materials were contaminated with about 10(5) plaque-forming units of the Wa strain of human rotavirus (HRV) suspended in faecal matter. The contaminated disks were then held for 10 days at either room temperature (22 +/- 2 degrees C) or 4 degrees C with the relative humidity (RH) at the high (85 +/- 5%), medium (50 +/- 5%) or low (25 +/- 5%) level. Survival was longer on non-porous surfaces at the lower temperature and at lower humidity. In contrast, survival on porous surfaces was very variable; better on cotton-polyester than on poster card or paper currency on which HRV survived very poorly. These results suggest that under the right environmental conditions, HRV-contaminated objects could play a role in the transmission of rotavirus infections in hospitals, nursing homes and day-care centres.

Full text

PDF
277

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brandt C. D., Kim H. W., Yolken R. H., Kapikian A. Z., Arrobio J. O., Rodriguez W. J., Wyatt R. G., Chanock R. M., Parrott R. H. Comparative epidemiology of two rotavirus serotypes and other viral agents associated with pediatric gastroenteritis. Am J Epidemiol. 1979 Sep;110(3):243–254. doi: 10.1093/oxfordjournals.aje.a112809. [DOI] [PubMed] [Google Scholar]
  2. Champsaur H., Henry-Amar M., Goldszmidt D., Prevot J., Bourjouane M., Questiaux E., Bach C. Rotavirus carriage, asymptomatic infection, and disease in the first two years of life. II. Serological response. J Infect Dis. 1984 May;149(5):675–682. doi: 10.1093/infdis/149.5.675. [DOI] [PubMed] [Google Scholar]
  3. Champsaur H., Questiaux E., Prevot J., Henry-Amar M., Goldszmidt D., Bourjouane M., Bach C. Rotavirus carriage, asymptomatic infection, and disease in the first two years of life. I. Virus shedding. J Infect Dis. 1984 May;149(5):667–674. doi: 10.1093/infdis/149.5.667. [DOI] [PubMed] [Google Scholar]
  4. Davidson G. P., Bishop R. F., Townley R. R., Holmes I. H. Importance of a new virus in acute sporadic enteritis in children. Lancet. 1975 Feb 1;1(7901):242–246. doi: 10.1016/s0140-6736(75)91140-x. [DOI] [PubMed] [Google Scholar]
  5. Echeverria P., Burke D. S., Blacklow N. R., Cukor G., Charoenkul C., Yanggratoke S. Age-specific prevalence of antibody to rotavirus, Escherichia coli heat-labile enterotoxin, Norwalk virus, and hepatitis A virus in a rural community in Thailand. J Clin Microbiol. 1983 May;17(5):923–925. doi: 10.1128/jcm.17.5.923-925.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Flewett T. H. Rotavirus in the home and hospital nursery. Br Med J (Clin Res Ed) 1983 Aug 27;287(6392):568–569. doi: 10.1136/bmj.287.6392.568. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Grimwood K., Abbott G. D., Fergusson D. M., Jennings L. C., Allan J. M. Spread of rotavirus within families: a community based study. Br Med J (Clin Res Ed) 1983 Aug 27;287(6392):575–577. doi: 10.1136/bmj.287.6392.575. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Halvorsrud J., Orstavik I. An epidemic of rotavirus-associated gastroenteritis in a nursing home for the elderly. Scand J Infect Dis. 1980;12(3):161–164. doi: 10.3109/inf.1980.12.issue-3.01. [DOI] [PubMed] [Google Scholar]
  9. Hara M., Mukoyama J., Tsurahara T., Saito Y., Tagaya I. Letter: Duovirus in schoolchildren with gastroenteritis. Lancet. 1976 Feb 7;1(7954):311–311. doi: 10.1016/s0140-6736(76)91449-5. [DOI] [PubMed] [Google Scholar]
  10. Harris J. R., Cohen M. L., Lippy E. C. Water-related disease outbreaks in the United States, 1981. J Infect Dis. 1983 Oct;148(4):759–762. doi: 10.1093/infdis/148.4.759. [DOI] [PubMed] [Google Scholar]
  11. Haug K. W., Orstavik I., Kvelstad G. Rotavirus infections in families. A clinical and virological study. Scand J Infect Dis. 1978;10(4):265–269. doi: 10.3109/inf.1978.10.issue-4.02. [DOI] [PubMed] [Google Scholar]
  12. Hildreth C., Thomas M., Ridgway G. L. Rotavirus infection in an obstetric unit. Br Med J (Clin Res Ed) 1981 Jan 17;282(6259):231–231. [PMC free article] [PubMed] [Google Scholar]
  13. Holdaway M. D., Kalmakoff J., Schroeder B. A., Wright G. C., Todd B. A., Jennings L. C. Rotavirus infection in Otago: a serological study. N Z Med J. 1982 Feb 24;95(702):110–112. [PubMed] [Google Scholar]
  14. Holdaway M. D., Todd B. A., Schroeder B. A., Kalmakoff J. Rotavirus infection in New Zealand. N Z Med J. 1982 Feb 10;95(701):67–69. [PubMed] [Google Scholar]
  15. Holzel H., Cubitt D. W., McSwiggan D. A., Sanderson P. J., Church J. An outbreak of rotavirus infection among adults in a cardiology ward. J Infect. 1980 Mar;2(1):33–37. doi: 10.1016/s0163-4453(80)91750-8. [DOI] [PubMed] [Google Scholar]
  16. Hung T., Chen G. M., Wang C. G., Yao H. L., Fang Z. Y., Chao T. X., Chou Z. Y., Ye W., Chang X. J., Den S. S. Waterborne outbreak of rotavirus diarrhoea in adults in China caused by a novel rotavirus. Lancet. 1984 May 26;1(8387):1139–1142. [PubMed] [Google Scholar]
  17. Höh H., Presser W., Wigand R. Nosokomial-Infektion durch Rotaviren bei Erwachsenen. Dtsch Med Wochenschr. 1983 Oct 21;108(42):1586–1591. doi: 10.1055/s-2008-1069789. [DOI] [PubMed] [Google Scholar]
  18. Ijaz M. K., Sattar S. A., Johnson-Lussenburg C. M., Springthorpe V. S. Comparison of the airborne survival of calf rotavirus and poliovirus type 1 (Sabin) aerosolized as a mixture. Appl Environ Microbiol. 1985 Feb;49(2):289–293. doi: 10.1128/aem.49.2.289-293.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Keswick B. H., Pickering L. K., DuPont H. L., Woodward W. E. Prevalence of rotavirus in children in day care centers. J Pediatr. 1983 Jul;103(1):85–86. doi: 10.1016/s0022-3476(83)80785-9. [DOI] [PubMed] [Google Scholar]
  20. Keswick B. H., Pickering L. K., DuPont H. L., Woodward W. E. Survival and detection of rotaviruses on environmental surfaces in day care centers. Appl Environ Microbiol. 1983 Oct;46(4):813–816. doi: 10.1128/aem.46.4.813-816.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Kim H. W., Brandt C. D., Kapikian A. Z., Wyatt R. G., Arrobio J. O., Rodriguez W. J., Chanock R. M., Parrott R. H. Human reovirus-like agent infection. Occurrence in adult contacts of pediatric patients with gastroenteritis. JAMA. 1977 Aug 1;238(5):404–407. doi: 10.1001/jama.238.5.404. [DOI] [PubMed] [Google Scholar]
  22. Konno T., Suzuki H., Katsushima N., Imai A., Tazawa F., Kutsuzawa T., Kitaoka S., Sakamoto M., Yazaki N., Ishida N. Influence of temperature and relative humidity on human rotavirus infection in Japan. J Infect Dis. 1983 Jan;147(1):125–128. doi: 10.1093/infdis/147.1.125. [DOI] [PubMed] [Google Scholar]
  23. Lycke E., Blomberg J., Berg G., Eriksson A., Madsen L. Epidemic acute diarrhoea in adults associated with infantile gastroenteritis virus. Lancet. 1978 Nov 11;2(8098):1056–1057. doi: 10.1016/s0140-6736(78)92389-9. [DOI] [PubMed] [Google Scholar]
  24. Marrie T. J., Lee S. H., Faulkner R. S., Ethier J., Young C. H. Rotavirus infection in a geriatric population. Arch Intern Med. 1982 Feb;142(2):313–316. [PubMed] [Google Scholar]
  25. McNulty M. S., Logan E. F. Longitudinal survey of rotavirus infection in calves. Vet Rec. 1983 Oct 8;113(15):333–335. doi: 10.1136/vr.113.15.333. [DOI] [PubMed] [Google Scholar]
  26. Middleton P. J., Szymanski M. T., Petric M. Viruses associated with acute gastroenteritis in young children. Am J Dis Child. 1977 Jul;131(7):733–737. doi: 10.1001/archpedi.1977.02120200015004. [DOI] [PubMed] [Google Scholar]
  27. Moe K., Shirley J. A. The effects of relative humidity and temperature on the survival of human rotavirus in faeces. Arch Virol. 1982;72(3):179–186. doi: 10.1007/BF01348963. [DOI] [PubMed] [Google Scholar]
  28. Morens D. M., Zweighaft R. M., Vernon T. M., Gary G. W., Eslien J. J., Wood B. T., Holman R. C., Dolin R. A waterborne outbreak of gastroenteritis with secondary person-to-person spread. Association with a viral agent. Lancet. 1979 May 5;1(8123):964–966. doi: 10.1016/s0140-6736(79)91734-3. [DOI] [PubMed] [Google Scholar]
  29. Murphy A. M., Albrey M. B., Crewe E. B. Rotavirus infections of neonates. Lancet. 1977 Dec 3;2(8049):1149–1150. doi: 10.1016/S0140-6736(77)91538-0. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Noone C., Banatvala J. E. Hospital acquired rotaviral gastroenteritis in a general paediatric unit. J Hosp Infect. 1983 Sep;4(3):297–299. doi: 10.1016/0195-6701(83)90031-2. [DOI] [PubMed] [Google Scholar]
  31. Pickering L. K., Evans D. G., DuPont H. L., Vollet J. J., 3rd, Evans D. J., Jr Diarrhea caused by Shigella, rotavirus, and Giardia in day-care centers: prospective study. J Pediatr. 1981 Jul;99(1):51–56. doi: 10.1016/s0022-3476(81)80956-0. [DOI] [PubMed] [Google Scholar]
  32. Prince A. M., Brotman B., Grady G. F., Kuhns W. J., Hazzi C., Levine R. W., Millian S. J. Long-incubation post-transfusion hepatitis without serological evidence of exposure to hepatitis-B virus. Lancet. 1974 Aug 3;2(7875):241–246. doi: 10.1016/s0140-6736(74)91412-3. [DOI] [PubMed] [Google Scholar]
  33. Ramia S., Sattar S. A. Simian rotavirus SA-11 plaque formation in the presence of trypsin. J Clin Microbiol. 1979 Nov;10(5):609–614. doi: 10.1128/jcm.10.5.609-614.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Rochi G. U., Vella S., Resta S., Cochi S., Donelli G., Tangucci F., Menichella D., Varveri A., Inglese R. Outbreak of rotavirus gastroenteritis among premature infants. Br Med J (Clin Res Ed) 1981 Oct 3;283(6296):886–886. doi: 10.1136/bmj.283.6296.886. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Ryder R. W., McGowan J. E., Hatch M. H., Palmer E. L. Reovirus-like agent as a cause of nosocomial diarrhea in infants. J Pediatr. 1977 May;90(5):698–702. doi: 10.1016/s0022-3476(77)81230-4. [DOI] [PubMed] [Google Scholar]
  36. Samadi A. R., Huq M. I., Ahmed Q. S. Detection of rotavirus in handwashings of attendants of children with diarrhoea. Br Med J (Clin Res Ed) 1983 Jan 15;286(6360):188–188. doi: 10.1136/bmj.286.6360.188. [DOI] [PMC free article] [PubMed] [Google Scholar]
  37. Sattar S. A., Ijaz M. K., Johnson-Lussenburg C. M., Springthorpe V. S. Effect of relative humidity on the airborne survival of rotavirus SA11. Appl Environ Microbiol. 1984 Apr;47(4):879–881. doi: 10.1128/aem.47.4.879-881.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  38. Sattar S. A., Raphael R. A., Lochnan H., Springthorpe V. S. Rotavirus inactivation by chemical disinfectants and antiseptics used in hospitals. Can J Microbiol. 1983 Oct;29(10):1464–1469. doi: 10.1139/m83-225. [DOI] [PubMed] [Google Scholar]
  39. Sutmoller F., Azeredo R. S., Lacerda M. D., Barth O. M., Pereira H. G., Hoffer E., Schatzmayr H. G. An outbreak of gastroenteritis caused by both rotavirus and Shigella sonnei in a private school in Rio de Janeiro. J Hyg (Lond) 1982 Apr;88(2):285–293. doi: 10.1017/s0022172400070145. [DOI] [PMC free article] [PubMed] [Google Scholar]
  40. Tan J. A., Schnagl R. D. Inactivation of a rotavirus by disinfectants. Med J Aust. 1981 Jan 10;1(1):19–23. doi: 10.5694/j.1326-5377.1981.tb135281.x. [DOI] [PubMed] [Google Scholar]
  41. Totterdell B. M., Chrystie I. L., Banatvala J. E. Rotavirus infections in a maternity unit. Arch Dis Child. 1976 Dec;51(12):924–928. doi: 10.1136/adc.51.12.924. [DOI] [PMC free article] [PubMed] [Google Scholar]
  42. Woode G. N., Bridger J. C. Viral enteritis of calves. Vet Rec. 1975 Jan 25;96(4):85–88. doi: 10.1136/vr.96.4.85. [DOI] [PubMed] [Google Scholar]
  43. Wyatt R. G., James W. D., Bohl E. H., Theil K. W., Saif L. J., Kalica A. R., Greenberg H. B., Kapikian A. Z., Chanock R. M. Human rotavirus type 2: cultivation in vitro. Science. 1980 Jan 11;207(4427):189–191. doi: 10.1126/science.6243190. [DOI] [PubMed] [Google Scholar]

Articles from The Journal of Hygiene are provided here courtesy of Cambridge University Press

RESOURCES