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. 1986 Jun;96(3):377–384. doi: 10.1017/s0022172400066134

Monoclonal antibodies directed against the flagella of Campylobacter jejuni: cross-reacting and serotypic specificity and potential use in diagnosis.

D G Newell
PMCID: PMC2129687  PMID: 3734423

Abstract

Nine monoclonal antibodies directed against the flagella of Campylobacter jejuni strain 81116 have been investigated for serotypic and cross-reacting activity using a panel of 17 Penner serotype strains of C. jejuni. Four monoclonal antibodies were exclusively specific for serotype-6 strains, which was the serotype of C. jejuni strain 81116. Two monoclonal antibodies cross-reacted with all the flagellated strains of C. jejuni tested. One of these cross-reacting monoclonal antibodies, CF5, was found to react with all other Campylobacter species except C. sputorum bulbulus but it did not react with other bacterial enteropathogens. An antigen-capture ELISA technique was established, using this monoclonal antibody, which could detect flagellar antigen in human faecal material. These anti-flagella monoclonal antibodies therefore may be valuable in the diagnosis and serotyping of C. jejuni in clinical material.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Hawkes R., Niday E., Gordon J. A dot-immunobinding assay for monoclonal and other antibodies. Anal Biochem. 1982 Jan 1;119(1):142–147. doi: 10.1016/0003-2697(82)90677-7. [DOI] [PubMed] [Google Scholar]
  2. Jones D. M., Curry A., Fox A. J. An ultrastructural study of the gastric campylobacter-like organism 'Campylobacter pyloridis'. J Gen Microbiol. 1985 Sep;131(9):2335–2341. doi: 10.1099/00221287-131-9-2335. [DOI] [PubMed] [Google Scholar]
  3. Kaijser B., Sjögren E. Campylobacter strains in Sweden. Serotyping and correlation to clinical symptoms. Acta Pathol Microbiol Immunol Scand B. 1985 Aug;93(4):315–322. [PubMed] [Google Scholar]
  4. Morooka T., Umeda A., Amako K. Motility as an intestinal colonization factor for Campylobacter jejuni. J Gen Microbiol. 1985 Aug;131(8):1973–1980. doi: 10.1099/00221287-131-8-1973. [DOI] [PubMed] [Google Scholar]
  5. Nachamkin I., Hart A. M. Western blot analysis of the human antibody response to Campylobacter jejuni cellular antigens during gastrointestinal infection. J Clin Microbiol. 1985 Jan;21(1):33–38. doi: 10.1128/jcm.21.1.33-38.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Newell D. G., McBride H., Dolby J. M. Investigations on the role of flagella in the colonization of infant mice with Campylobacter jejuni and attachment of Campylobacter jejuni to human epithelial cell lines. J Hyg (Lond) 1985 Oct;95(2):217–227. doi: 10.1017/s0022172400062653. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Newell D. G., McBride H., Pearson A. D. The identification of outer membrane proteins and flagella of Campylobacter jejuni. J Gen Microbiol. 1984 May;130(5):1201–1208. doi: 10.1099/00221287-130-5-1201. [DOI] [PubMed] [Google Scholar]
  8. Patton C. M., Barrett T. J., Morris G. K. Comparison of the Penner and Lior methods for serotyping Campylobacter spp. J Clin Microbiol. 1985 Oct;22(4):558–565. doi: 10.1128/jcm.22.4.558-565.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Thomas K., Chan K. N., Ribeiro C. D. Campylobacter jejuni/coli meningitis in a neonate. Br Med J. 1980 May 31;280(6227):1301–1302. doi: 10.1136/bmj.280.6227.1301. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Wenman W. M., Chai J., Louie T. J., Goudreau C., Lior H., Newell D. G., Pearson A. D., Taylor D. E. Antigenic analysis of Campylobacter flagellar protein and other proteins. J Clin Microbiol. 1985 Jan;21(1):108–112. doi: 10.1128/jcm.21.1.108-112.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]

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