Abstract
Antisera against live cultures of 20 strains of Bacteroides fragilis produced in rabbits agglutinated homologous whole-cell as well as O-antigen suspensions with several cross-reactions. Pure specific antisera were produced by absorbing cross-reacting antigens. O-antisera reacted against O-antigens but failed to do so against most whole-cell suspensions, suggesting the presence of surface structures which might have blocked the reactions. The titres of the whole-cell antisera were not significantly higher than those of O-antisera, but the reactions were more definite and easier to read. This system may prove useful in serological studies of B. fragilis.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Beerens H., Wattre P., Shinjo T., Romond C. Premiers résultats d'un essai de classification sérologique de 131 souches de Bacteroïdes du groupe fragilis (eggerthella. Ann Inst Pasteur (Paris) 1971 Aug;121(2):187–198. [PubMed] [Google Scholar]
- Elhag K. M., Bettelheim K. A., Tabaqchali S. Serological studies of Bacteroides fragilis. J Hyg (Lond) 1977 Oct;79(2):233–241. doi: 10.1017/s0022172400053043. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kasper D. L., Seiler M. W. Immunochemical characterization of the outer membrane complex of Bacteroides fragilis subspecies fragilis. J Infect Dis. 1975 Oct;132(4):440–450. doi: 10.1093/infdis/132.4.440. [DOI] [PubMed] [Google Scholar]
- Kasper D. L. The polysaccharide capsule of Bacteroides fragilis subspecies fragilis: immunochemical and morphologic definition. J Infect Dis. 1976 Jan;133(1):79–87. doi: 10.1093/infdis/133.1.79. [DOI] [PubMed] [Google Scholar]
- Lambe D. W., Jr, Moroz D. A. Serogrouping of Bacteroides fragilis subsp. fragilis by the agglutination test. J Clin Microbiol. 1976 Jun;3(6):586–592. doi: 10.1128/jcm.3.6.586-592.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sharpe M. E. Serology of rumen bacteroides. J Gen Microbiol. 1971 Aug;67(3):273–288. doi: 10.1099/00221287-67-3-273. [DOI] [PubMed] [Google Scholar]
- Starr S. E., Thompson F. S., Dowell V. R., Jr, Balows A. Micromethod system for identification of anaerobic bacteria. Appl Microbiol. 1973 May;25(5):713–717. doi: 10.1128/am.25.5.713-717.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]