Skip to main content
NCBI home page
The Journal of Hygiene logoLink to The Journal of Hygiene
. 1978 Jun;80(3):391–399. doi: 10.1017/s0022172400024840

Enzyme-labelled immunosorbent assay techniques in foot-and-mouth disease virus research.

E M Abu Elzein, J R Crowther
PMCID: PMC2129796  PMID: 206625

Abstract

The indirect ELISA technique has been developed successfully to measure antibodies to foot-and-mouth disease virus (FMDV) in cattle sera. Preliminary studies using a standard serum assay show that reproducible results are obtained. The method should prove useful for the examination of antibody titres in sera from large numbers of cattle or other animals.

Full text

PDF
391

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Avrameas S. Coupling of enzymes to proteins with glutaraldehyde. Use of the conjugates for the detection of antigens and antibodies. Immunochemistry. 1969 Jan;6(1):43–52. doi: 10.1016/0019-2791(69)90177-3. [DOI] [PubMed] [Google Scholar]
  2. BROWN F., CARTWRIGHT B. PURIFICATION OF RADIOACTIVE FOOT-AND-MOUTH DISEASE VIRUS. Nature. 1963 Sep 21;199:1168–1170. doi: 10.1038/1991168a0. [DOI] [PubMed] [Google Scholar]
  3. Engvall E., Perlmann P. Enzyme-linked immunosorbent assay (ELISA). Quantitative assay of immunoglobulin G. Immunochemistry. 1971 Sep;8(9):871–874. doi: 10.1016/0019-2791(71)90454-x. [DOI] [PubMed] [Google Scholar]
  4. Golding S. M., Hedger R. S., Talbot P. Radial immuno-diffusion and serum-neutralisation techniques for the assay of antibodies to swine vesicular disease. Res Vet Sci. 1976 Mar;20(2):142–147. [PubMed] [Google Scholar]
  5. Harris T. J., Brown F. Biochemical analysis of a virulent and an avirulent strain of foot-and-mouth disease virus. J Gen Virol. 1977 Jan;34(1):87–105. doi: 10.1099/0022-1317-34-1-87. [DOI] [PubMed] [Google Scholar]
  6. Nakane P K, Pierce G B., Jr Enzyme-labeled antibodies: preparation and application for the localization of antigens. J Histochem Cytochem. 1966 Dec;14(12):929–931. doi: 10.1177/14.12.929. [DOI] [PubMed] [Google Scholar]
  7. Van Weemen B. K., Schuurs A. H.W.M. Immunoassay using antigen-enzyme conjugates. FEBS Lett. 1971 Jun 24;15(3):232–236. doi: 10.1016/0014-5793(71)80319-8. [DOI] [PubMed] [Google Scholar]
  8. Voller A., Bidwell D. E. Enzyme-immunoassays for antibodies in measles, cytomegalovirus infections and after rubella vaccination. Br J Exp Pathol. 1976 Apr;57(2):243–247. [PMC free article] [PubMed] [Google Scholar]
  9. Voller A., Bidwell D., Bartlett A. A serological study on human Trypanosoma rhodesiense infections using a micro-scale enzyme linked immunosorbent assay. Tropenmed Parasitol. 1975 Jun;26(2):247–251. [PubMed] [Google Scholar]
  10. Voller A., Bidwell D., Huldt G., Engvall E. A microplate method of enzyme-linked immunosorbent assay and its application to malaria. Bull World Health Organ. 1974;51(2):209–211. [PMC free article] [PubMed] [Google Scholar]
  11. Wicker R., Avrameas S. Localization of virus antigens by enzyme-labelled antibodies. J Gen Virol. 1969 Jun;4(4):465–471. doi: 10.1099/0022-1317-4-4-465. [DOI] [PubMed] [Google Scholar]
  12. Wolters G., Kuijpers L., Kacaki J., Schuurs A. Solid-phase enzyme-immunoassay for detection of hepatitis B surface antigen. J Clin Pathol. 1976 Oct;29(10):873–879. doi: 10.1136/jcp.29.10.873. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Wood B. T., Thompson S. H., Goldstein G. Fluorescent antibody staining. 3. Preparation of fluorescein-isothiocyanate-labeled antibodies. J Immunol. 1965 Aug;95(2):225–229. [PubMed] [Google Scholar]
  14. Yolken R. H., Kim H. W., Clem T., Wyatt R. G., Kalica A. R., Chanock R. M., Kapikian A. Z. Enzyme-linked immunosorbent assay (ELISA) for detection of human reovirus-like agent of infantile gastroenteritis. Lancet. 1977 Aug 6;2(8032):263–267. doi: 10.1016/s0140-6736(77)90951-5. [DOI] [PubMed] [Google Scholar]
  15. van Weemen B. K., Schuurs A. H. Immunoassay using hapten--enzyme conjugates. FEBS Lett. 1972 Jul 15;24(1):77–81. doi: 10.1016/0014-5793(72)80830-5. [DOI] [PubMed] [Google Scholar]

Articles from The Journal of Hygiene are provided here courtesy of Cambridge University Press

RESOURCES