Abstract
A modification of Sharpe's microdroplet counting technique is presented which permits viable counting of Bacteroides fragilis. Lysed horse blood was added to 0.1 soy digest agar droplets and 2% was found to be the optimal concentration which combined adequate intradroplet colony size with acceptable visibility on the counting screen of the Colworth Droplette machine.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Edlich R. F., Rogers W., Kasper G., Kaufman D., Tsung M. S., Wangensteen O. H. Studies in the management of the contaminated wound. I. Optimal time for closure of contaminated open wounds. II. Comparison of resistance to infection of open and closed wounds during healing. Am J Surg. 1969 Mar;117(3):323–329. doi: 10.1016/0002-9610(69)90365-1. [DOI] [PubMed] [Google Scholar]
- Kelly M. J. Aerobic and anaerobic mixtures of human pathogens: a rapid 4-plate counting technique. Br J Exp Pathol. 1977 Oct;58(5):478–483. [PMC free article] [PubMed] [Google Scholar]
- Lari J., Kirk D., Howden R. Bacteriological survey of acute appendicitis in children. Br J Surg. 1976 Aug;63(8):643–646. doi: 10.1002/bjs.1800630821. [DOI] [PubMed] [Google Scholar]
- Leigh D. A. Clinical importance of infections due to Bacteroides fragilis and role of antibiotic therapy. Br Med J. 1974 Jul 27;3(5925):225–228. doi: 10.1136/bmj.3.5925.225. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nobles E. R., Jr Bacteroides infections. Ann Surg. 1973 May;177(5):601–606. [PMC free article] [PubMed] [Google Scholar]
- Sharpe A. N., Dyett E. J., Jackson A. K., Kilsby D. C. Technique and apparatus for rapid and inexpensive enumeration of bacteria. Appl Microbiol. 1972 Jul;24(1):4–7. doi: 10.1128/am.24.1.4-7.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sharpe A. N., Kilsby D. C. A rapid, inexpensive bacterial count technique using agar droplets. J Appl Bacteriol. 1971 Jun;34(2):435–440. doi: 10.1111/j.1365-2672.1971.tb02303.x. [DOI] [PubMed] [Google Scholar]