Abstract
A comparison was made between a macrotechnique in tubes and a microtechnique in plastic plates for complement-fixation tests, using strains of three subtypes of the Asia 1 type of foot-and-mouth disease (FMD) virus. The results obtained with these techniques were found to be comparable and delineated the antigenic relationships of the three strains employed. The microtechnique was considered to be both economical with reagents and capable of similar accuracy and reproducibility to that of the standard method in tubes. It was concluded that the antigenic analysis of subtype strains of FMD virus can be conveniently carried out by the use of the microtechnique as described.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- ARCHETTI I., HORSFALL F. L., Jr Persistent antigenic variation of influenza A viruses after incomplete neutralization in ovo with heterologous immune serum. J Exp Med. 1950 Nov 1;92(5):441–462. doi: 10.1084/jem.92.5.441. [DOI] [PMC free article] [PubMed] [Google Scholar]
- BRADISH C. J., BROOKSBY J. B. Complement-fixation studies of the specificity of the interactions between components of the virus system of foot-and-mouth disease and its antibodies. J Gen Microbiol. 1960 Apr;22:405–415. doi: 10.1099/00221287-22-2-405. [DOI] [PubMed] [Google Scholar]
- BRADISH C. J., BROOKSBY J. B., TSUBAHARA H. The complement-fixation test in studies of components of the virus system of foot-and-mouth disease and its antibodies. J Gen Microbiol. 1960 Apr;22:392–404. doi: 10.1099/00221287-22-2-392. [DOI] [PubMed] [Google Scholar]
- CHU C. M., ANDREWES C. H., GLEDHILL A. W. Influenza in 1948-1949. Bull World Health Organ. 1950;3(2):187–214. [PMC free article] [PubMed] [Google Scholar]
- DAVIE J. A COMPLEMENT FIXATION TECHNIQUE FOR THE QUANTITATIVE MEASUREMENT OF ANTIGENIC DIFFERENCES BETWEEN STRAINS OF THE VIRUS OF FOOT-AND-MOUTH DISEASE. J Hyg (Lond) 1964 Dec;62:401–411. doi: 10.1017/s0022172400040146. [DOI] [PMC free article] [PubMed] [Google Scholar]
- GRAVES J. H. The differentiation of subtypes (variants) of foot-and-mouth disease virus by serologic methods. I. Complement-fixation test. Am J Vet Res. 1960 Jul;21:687–690. [PubMed] [Google Scholar]
- JORDAN W. S., Jr, GAYLIN S. G. The antigenic variation of influenza B viruses; demonstration of an antigenic spectrum by use of titer ratios. J Immunol. 1953 Apr;70(4):393–399. [PubMed] [Google Scholar]
- KAMITSUKA P., LENAHAN M., WENNER H. A. A comparative study of type 2 poliomyelitis viruses. II. Antigenic differences relating to 18 type 2 strains. J Immunol. 1956 Oct;77(4):220–231. [PubMed] [Google Scholar]
- KIPPS A. Complement fixation with antigens prepared from bluetongue virus-infected mouse brains. J Hyg (Lond) 1956 Mar;54(1):79–88. doi: 10.1017/s0022172400044326. [DOI] [PMC free article] [PubMed] [Google Scholar]