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. 1979 Dec;83(3):501–505. doi: 10.1017/s0022172400026346

The effect of an intramammary infusion of endotoxin on experimentally induced mycoplasmal mastitis.

J Brownlie, C J Howard, R N Gourlay
PMCID: PMC2130171  PMID: 390046

Abstract

The infusion of 10 microgram of endotoxin lipopolysaccharide from Escherichia coli into the mammary gland of four cows 16 h before inoculation with ureaplasmas did not prevent, or even diminish, the subsequent ureaplasmal mastitis. There was no reduction in the severity or duration of the inflammatory cell response in milk or in the clinical appearance of the resulting mastitis. Also, the excretion of ureaplasmas was not reduced. A similar experiment with Mycoplasma dispar in two cows demonstrated that endotoxin was again ineffective in preventing the mastitis. Furthermore, there was some indication that the proliferation and excretion of this mycoplasma was enhanced in endotoxin-treated quarters.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andrews B. E., Leach R. H., Gourlay R. N., Howard C. J. Enhanced isolation of Mycoplasma dispar by substitution of ampicillin for benzylpenicillin in growth media. Vet Rec. 1973 Dec 8;93(23):603–603. doi: 10.1136/vr.93.23.603-a. [DOI] [PubMed] [Google Scholar]
  2. BLOBEL H., KATSUBE Y. EFFECTS OF EXPERIMENTALLY INDUCED LEUKOCYTOSIS IN BOVINE MAMMARY GLANDS UPON INFECTIONS WITH STAPHYLOCOCCUS AUREUS, STREPTOCOCCUS AGALACTIAE, AND AEROBACTER AEROGENES. Am J Vet Res. 1964 Jul;25:1085–1089. [PubMed] [Google Scholar]
  3. Belham J. E., Neal G. E. Testosterone action in the rat ventral prostate. The effects of diethylstilboestrol and cyproterone acetate on the metabolism of ( 3 H)testosterone and the retention of labelled metabolites by rat ventral prostate in vivo and in vitro. Biochem J. 1971 Nov;125(1):81–91. doi: 10.1042/bj1250081. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Brownlie J., Howard C. J., Gourlay R. N. Mycoplasmacidal activity of bovine milk for T-mycoplasmas. J Hyg (Lond) 1974 Dec;73(3):415–423. doi: 10.1017/s0022172400042777. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Brownlie J., Howard C. J., Gourlay R. N. Pathogenicity of certain Mycoplasma species in the bovine mammary gland. Res Vet Sci. 1976 May;20(3):261–266. [PubMed] [Google Scholar]
  6. Brownlie J. The effect of an intramammary infusion of endotoxin on the establishment of experimental mastitis by Streptococcus agalactiae in the cow. J Hyg (Lond) 1979 Aug;83(1):103–109. doi: 10.1017/s0022172400025869. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. DERBYSHIRE J. B. THE MULTIPLICATION OF STAPHYLOCOCCUS AUREUS IN THE MILK OF COWS WITH MILD MASTITIS. J Pathol Bacteriol. 1964 Jan;87:137–144. doi: 10.1002/path.1700870119. [DOI] [PubMed] [Google Scholar]
  8. Gourlay R. N., Howard C. J., Brownlie J. The production of mastitis in cows by the intramammary inoculation of T-mycoplasmas. J Hyg (Lond) 1972 Sep;70(3):511–521. doi: 10.1017/s0022172400063099. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Gourlay R. N., Leach R. H. A new mycoplasma species isolated from pneumonic lungs of calves (Mycoplasma dispar sp. nov.). J Med Microbiol. 1970 Feb;3(1):111–123. doi: 10.1099/00222615-3-1-111. [DOI] [PubMed] [Google Scholar]
  10. Howard C. J., Brownlie J., Gourlay R. N., Collins J. Presence of a dialysable fraction in normal bovine whey capable of killing several species of bovine mycoplasmas. J Hyg (Lond) 1975 Apr;74(2):261–270. doi: 10.1017/s0022172400024335. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Howard C. J., Gourlay R. N., Brownlie J. The virulence of T-mycoplasmas, isolated from various animal species, assayed by intramammary inoculation in cattle. J Hyg (Lond) 1973 Mar;71(1):163–170. doi: 10.1017/s0022172400046337. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Howard C. J., Taylor G., Collins J., Gourlay R. N. Interaction of Mycoplasma dispar and Mycoplasma agalactiae subsp. bovis with bovine alveolar macrophages and bovine lacteal polymorphonuclear leukocytes. Infect Immun. 1976 Jul;14(1):11–17. doi: 10.1128/iai.14.1.11-17.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Jain N. C., Jasper D. E., Dellinger J. D. Experimental bovine mastitis due to mycoplasma. Cornell Vet. 1969 Jan;59(1):10–28. [PubMed] [Google Scholar]
  14. Paape M. J., Wergin W. P. The leukocyte as a defense mechanism. J Am Vet Med Assoc. 1977 May 15;170(10 Pt 2):1214–1223. [PubMed] [Google Scholar]

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