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. 1975 Aug;75(1):99–112. doi: 10.1017/s0022172400047112

Detection of endotoxins with the Limulus test in burned and unburned mice infected with different species of gram-negative bacteria.

R J Jones, E A Roe, R E Dyster
PMCID: PMC2130240  PMID: 807621

Abstract

The Limulus test detected endotoxins in the plasma of burned and unburned mice infected with different species of gram-negative bacteria produced different amounts of endotoxin in the plasma of infected mice. Plasma from mice given lethal infections showed very high concentrations of endotoxin. Low concentrations of endotoxin in the plasma were tolerated by mice but high concentrations were invariably fatal. A polyvalent pseudomonas vaccine reduced endotoxin in the plasma of mice given lethal infections of Pseudomonas aeruginosa.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Caridis D. T., Reinhold R. B., Woodruff P. W., Fine J. Endotoxaemia in man. Lancet. 1972 Jun 24;1(7765):1381–1385. doi: 10.1016/s0140-6736(72)91108-7. [DOI] [PubMed] [Google Scholar]
  2. Cuevas P., Ishiyama M., Koizumi S., Woodruff P., Kaufman A., Fine J. Role of endotoxemia of intestinal origin in early death from large burns. Surg Gynecol Obstet. 1974 May;138(5):725–730. [PubMed] [Google Scholar]
  3. Davis B., Lilly H. A., Lowbury E. J. Gram-negative bacilli in burns. J Clin Pathol. 1969 Nov;22(6):634–641. doi: 10.1136/jcp.22.6.634. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Jones R. J., Dyster R. E. The role of polymorphonuclear leucocytes in protecting mice vaccinated against Pseudomon as aeruginosa infections. Br J Exp Pathol. 1973 Aug;54(4):416–421. [PMC free article] [PubMed] [Google Scholar]
  5. Jones R. J., Jackson D. M., Lowbury E. J. Antiserum and antibiotic in the prophylaxis of burns against Pseudomonas aeruginosa. Br J Plast Surg. 1966 Jan;19(1):43–57. doi: 10.1016/s0007-1226(66)80007-3. [DOI] [PubMed] [Google Scholar]
  6. Jones R. J., Lowbury E. J. Early protection by vaccines against Pseudomonas aeruginosa colonizing burns. Br J Exp Pathol. 1972 Dec;53(6):659–664. [PMC free article] [PubMed] [Google Scholar]
  7. Levin J., Bang F. B. Clottable protein in Limulus; its localization and kinetics of its coagulation by endotoxin. Thromb Diath Haemorrh. 1968 Mar 31;19(1):186–197. [PubMed] [Google Scholar]
  8. Lowbury E. J. Infection associated with burns. Postgrad Med J. 1972 Jun;48(560):338–341. [PMC free article] [PubMed] [Google Scholar]
  9. Reinhold R. B., Fine J. A technique for quantitative measurement of endotoxin in human plasma. Proc Soc Exp Biol Med. 1971 May;137(1):334–340. doi: 10.3181/00379727-137-35572. [DOI] [PubMed] [Google Scholar]
  10. Yin E. T., Galanos C., Kinsky S., Bradshaw R. A., Wessler S., Lüderitz O., Sarmiento M. E. Picogram-sensitive assay for endotoxin: gelation of Limulus polyphemus blood cell lysate induced by purified lipopolysaccharides and lipid A from Gram-negative bacteria. Biochim Biophys Acta. 1972 Jan 28;261(1):284–289. doi: 10.1016/0304-4165(72)90340-6. [DOI] [PubMed] [Google Scholar]

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