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. 1974 Oct;73(2):287–295. doi: 10.1017/s0022172400024141

Use of single radial immunodiffusion test for serological studies in volunteers inoculated with live attenuated influenza virus

M E Molyneux, A S Beare, K Callow, G C Schild
PMCID: PMC2130313  PMID: 4214175

Abstract

Pre- and post-vaccination serum samples from 278 volunteers, who were given live influenza vaccines, were tested by haemagglutination inhibition (HI) and single radial immunodiffusion tests(SRDT) for antibody to influenza A/Hong Kong/1/68 (H3N2) virus envelope antigens. Those with high antibody titres detected in both tests were less frequently infected, and 85% of the 159 infected showed rises by HI and 70% by SRDT. Similarly, 69 pairs were tested for antibody to Hong Kong (N2) neuraminidase by neuraminidase inhibition test (NI) and by SRD tests. Those with high titres in both tests resisted infection and those who were infected showed a rise in antibody detected both by NI and SRD tests. In general, SRDT was less sensitive than HI and NI in detecting antibody and antibody rises, but in some volunteers it did detect antibody rises which were not detected by conventional tests. Because of its simplicity and speed it appeared to be of use in evaluating such vaccines.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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