Abstract
A number of outbreaks of food poisoning attributed to Bacillus cereus have been reported recently and all have been associated with cooked rice usually from Chinese restaurants and `take-away' shops.
Tests were made to assess the heat resistance of B. cereus spores in aqueous suspension, the growth of the organism in boiled rice stored at temperatures in the range 4-55° C., and the effect of cooking and storage on the growth of the organism in boiled and fried rice. The spores of B. cereus survived cooking and were capable of germination and outgrowth. The optimum temperature for growth in boiled rice was between 30° and 37° C. and growth also occurred during storage at 15° and 43° C.
To prevent further outbreaks it is suggested that rice should be boiled in smaller quantities on several occasions during the day, thereby reducing the storage time before frying. After boiling the rice should either be kept hot (> 63° C.) or cooled quickly and transferred to a refrigerator within 2 hr. of cooking. Boiled or fried rice must not be stored under warm conditions especially in the range 15-50° C.
Full text
PDFSelected References
These references are in PubMed. This may not be the complete list of references from this article.
- Briggs A. The resistances of spores of the genus Bacillus to phenol, heat and radiation. J Appl Bacteriol. 1966 Dec;29(3):490–504. doi: 10.1111/j.1365-2672.1966.tb03500.x. [DOI] [PubMed] [Google Scholar]
- GORDON R. E., SMITH M. M. Proposed group of characters for the separation of Streptomyces and Nocardia. J Bacteriol. 1955 Feb;69(2):147–150. doi: 10.1128/jb.69.2.147-150.1955. [DOI] [PMC free article] [PubMed] [Google Scholar]
- JANTEA F., MILOSESCU P., BISTRICEANU E., BAD-OPRISESCU D. INCIDEN TA B. CEREUS IN PREPARATE CULINARE. Microbiol Parazitol Epidemiol (Bucur) 1965 Mar-Apr;10:163–168. [PubMed] [Google Scholar]
- LONG S. K., WILLIAMS O. B. Method for removal of vegetative cells from Bacterial spore preparations. J Bacteriol. 1958 Sep;76(3):332–332. doi: 10.1128/jb.76.3.332-332.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Molin N., Snygg B. G. Effect of lipid materials on heat resistance of bacterial spores. Appl Microbiol. 1967 Nov;15(6):1422–1426. doi: 10.1128/am.15.6.1422-1426.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mortimer P. R., McCann G. Food-poisoning episodes associated with Bacillus cereus in fried rice. Lancet. 1974 May 25;1(7865):1043–1045. doi: 10.1016/s0140-6736(74)90434-6. [DOI] [PubMed] [Google Scholar]
- Mossel D. A., Koopman M. J., Jongerius E. Enumeration of Bacillus cereus in foods. Appl Microbiol. 1967 May;15(3):650–653. doi: 10.1128/am.15.3.650-653.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Navani S. K., Scholefield J., Kibby M. R. A digital computer program for the statistical analysis of heat resistance data applied to Bacillus stearothermophilus spores. J Appl Bacteriol. 1970 Dec;33(4):609–620. doi: 10.1111/j.1365-2672.1970.tb02242.x. [DOI] [PubMed] [Google Scholar]