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. 1973 Jun;71(2):403–410. doi: 10.1017/s0022172400022841

Typing of Pseudomonas aeruginosa: comparison of the phage procedure with the pyocine technique

Ruth Bernstein-Ziv, Rose Mushin, Kurt Rabinowitz
PMCID: PMC2130483  PMID: 4198203

Abstract

Two hundred and sixty strains of Pseudomonas aeruginosa were isolated from patients of the Asaf Harofe Government Hospital. The strains were typed by phage technique and 64 of them were also typed according to pyocine sensitivity. The two methods proved complementary, and reduced the number of untypable strains. Phage typing was performed with both routine test dilution (RTD) and more concentrated phage suspensions. The most reliable results were obtained at 100 RTD.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. AYLIFFE G. A., LOWBURY E. J., HAMILTON J. G., SMALL J. M., ASHESHOV E. A., PARKER M. T. HOSPITAL INFECTION WITH PSEUDOMONAS AERUGINOSA IN NEUROSURGERY. Lancet. 1965 Aug 21;2(7408):365–368. doi: 10.1016/s0140-6736(65)90343-0. [DOI] [PubMed] [Google Scholar]
  2. Ayliffe G. A., Barry D. R., Lowbury E. J., Roper-Hall M. J., Walker W. M. Postoperative infection with Pseudomonas aeruginosa in an eye hospital. Lancet. 1966 May 21;1(7447):1113–1117. doi: 10.1016/s0140-6736(66)91135-4. [DOI] [PubMed] [Google Scholar]
  3. Bergan T. A new bacteriophage typing set for Pseudomonas aeruginosa. 2. Characterization and comparisons of new and previous typing sets. Acta Pathol Microbiol Scand B Microbiol Immunol. 1972;80(2):189–201. doi: 10.1111/j.1699-0463.1972.tb00149.x. [DOI] [PubMed] [Google Scholar]
  4. Farmer J. J., 3rd, Herman L. G. Epidemiological fingerprinting of Pseudomonas aeruginosa by the production of and sensitivity of pyocin and bacteriophage. Appl Microbiol. 1969 Nov;18(5):760–765. doi: 10.1128/am.18.5.760-765.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Fierer J., Taylor P. M., Gezon H. M. Pseudomonas aeruginosa epidemic traced to delivery-room resuscitators. N Engl J Med. 1967 May 4;276(18):991–996. doi: 10.1056/NEJM196705042761801. [DOI] [PubMed] [Google Scholar]
  6. GRABER C. D., LATTA R., VOGEL E. H., Jr, BRAME R. Bacteriophage grouping of Pseudomonas aeruginosa, with special emphasis on lysotypes occurring in infected burns. Am J Clin Pathol. 1962 Jan;37:54–62. doi: 10.1093/ajcp/37.1.54. [DOI] [PubMed] [Google Scholar]
  7. Gillies R. R., Govan J. R. Typing of Pseudomonas pyocyanea by pyocine production. J Pathol Bacteriol. 1966 Apr;91(2):339–345. doi: 10.1002/path.1700910207. [DOI] [PubMed] [Google Scholar]
  8. Govan J. R., Gillies R. R. Further studies in the pyocine typing of Pseudomonas pyocyanea. J Med Microbiol. 1969 Feb;2(1):17–25. doi: 10.1099/00222615-2-1-17. [DOI] [PubMed] [Google Scholar]
  9. HABS I. Untersuchungen über die O-Antigene von Pseudomonas aeruginosa. Z Hyg Infektionskr. 1957;144(3):218–228. [PubMed] [Google Scholar]
  10. Matsumoto H., Tazaki T., Kato T. Serological and pyocine types of Pseudomonas aeruginosa from various sources. Jpn J Microbiol. 1968 Mar;12(1):111–119. doi: 10.1111/j.1348-0421.1968.tb00373.x. [DOI] [PubMed] [Google Scholar]
  11. Moffet H. L., Williams T. Bacteria recovered from distilled water and inhalation therapy equipment. Am J Dis Child. 1967 Jul;114(1):7–12. doi: 10.1001/archpedi.1967.02090220013002. [DOI] [PubMed] [Google Scholar]
  12. SUTTER V. L., HURST V., FENNELL J. SEMISYNTHETIC MEDIUM FOR BACTERIOPHAGE TYPING OF PSEUDOMONAS. J Bacteriol. 1963 Dec;86:1354–1356. doi: 10.1128/jb.86.6.1354-1356.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Silverstein M. N., Ellefson R. D., Ahern E. J. The syndrome of the sea-blue histiocyte. N Engl J Med. 1970 Jan 1;282(1):1–4. doi: 10.1056/NEJM197001012820101. [DOI] [PubMed] [Google Scholar]
  14. Stone H. H., Kolb L. D. The evolution and spread of gentamicin-resistant Pseudomonads. J Trauma. 1971 Jul;11(7):586–589. doi: 10.1097/00005373-197107000-00009. [DOI] [PubMed] [Google Scholar]
  15. Tagg J. R., Mushin R. Epidemiology of Pseudomonas aeruginosa infection in hospitals. 1. Pyocine typing of Ps. aeruginosa. Med J Aust. 1971 Apr 17;1(16):847–852. [PubMed] [Google Scholar]
  16. WAHBA A. H. THE PRODUCTION AND INACTIVATION OF PYOCINES. J Hyg (Lond) 1963 Dec;61:431–441. doi: 10.1017/s0022172400021057. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. WILLIAMS R., WILLIAMS E. D., HYAMS D. E. Cross-infection with Pseudomonas pyocyanea. Lancet. 1960 Feb 13;1(7120):376–379. doi: 10.1016/s0140-6736(60)90301-9. [DOI] [PubMed] [Google Scholar]

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