Abstract
A survey was conducted in 1971 in healthy reptiles supplied to the Central Animal Laboratory of Nijmegen for experimental animal research. In order to determine which salmonella serotypes occur, and whether there are several serotypes per animal, several strains of each positive sample were typed.
It was found that 160 of 169 samples contained salmonellas or lactose-negative Arizonas or both, and 95 different serotypes were isolated.
Of 127 animals examined individually, 67 were carriers of more than one serotype, 42 animals having two types, 21 three types and 4 animals four types.
Three enrichment methods were compared. These were tetrathionate broth incubated at 37° C. (T37) and at 43° C. (T43), and selenite broth incubated at 37° C. (SB). All were incubated for 48 hr. before subculture on brilliant-green agar plates. The enrichment methods T37, T43 and SB produced 99, 125 and 123 positive samples respectively, when taken separately. The combinations of T37 and T43, T37 and SB, and T43 and SB produced 145, 142 and 150 positive samples respectively.
The yield of serotypes in comparable samples showed no difference between the three enrichment methods. With the use of two methods the yield increased by about 38% compared with one method, and the combination of three methods showed an increase in serotype yield of about 64% compared with one method. A distinct preference by serotypes for definite enrichment methods was not proved.
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Selected References
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- BOVRE K., SANDBU P. Salmonella-excreting tortoises in Oslo. Acta Pathol Microbiol Scand. 1959;46:339–342. doi: 10.1111/j.1699-0463.1959.tb01104.x. [DOI] [PubMed] [Google Scholar]
- BOYCOTT J. A., TAYLOR J., DOUGLAS S. H. Salmonella in tortoises. J Pathol Bacteriol. 1953 Apr;65(2):401–411. doi: 10.1002/path.1700650213. [DOI] [PubMed] [Google Scholar]
- Hinshaw W. R., McNeil E. Lizards as Carriers of Salmonella and Paracolon Bacteria. J Bacteriol. 1947 Jun;53(6):715–718. doi: 10.1128/jb.53.6.715-718.1947. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LEE P. E., MACKERRAS I. M. Salmonella infections of Australian native animals. Aust J Exp Biol Med Sci. 1955 Feb;33(1):117–125. doi: 10.1038/icb.1955.13. [DOI] [PubMed] [Google Scholar]
- MACKEY J. P. Salmonellosis in Dar es Salaam. East Afr Med J. 1955 Jan;32(1):1–6. [PubMed] [Google Scholar]
- ZWART P. Salmonella and Arizona infections in reptiles in the Netherlands. Antonie Van Leeuwenhoek. 1960;26:250–254. doi: 10.1007/BF02539011. [DOI] [PubMed] [Google Scholar]
- Zwart P., Poelma F. G., Strik W. J. The distribution of various types of Salmonellae and Arizonas in reptiles. Zentralbl Bakteriol Orig. 1970;213(2):201–212. [PubMed] [Google Scholar]
- de Hamel F. A., McInnes H. M. Lizards as vectors of human salmonellosis. J Hyg (Lond) 1971 Jun;69(2):247–253. doi: 10.1017/s0022172400021471. [DOI] [PMC free article] [PubMed] [Google Scholar]