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. 1970 Mar;68(1):81–96.2. doi: 10.1017/s0022172400028539

Soluble antigens obtained from influenza virus by treatment with non-ionic detergent

M J Corbel, C J M Rondle
PMCID: PMC2130777  PMID: 4986218

Abstract

Highly purified influenza virus was degraded using anionic and non-ionic detergents. Best results were obtained using the non-ionic detergent Triton N 101. Tests showed that virus extracts contained neuraminidase and a substance that reacted specifically with rabbit antibody to virus haemagglutinin (specific serum blocking substance). Haemagglutination-inhibiting antibody was produced when virus extracts were inoculated into guinea-pigs. Immunodiffusion tests showed that extracts were complex. Host-specific material was regularly found. Under appropriate conditions S-antigen was detected as a single line pattern component. Two or more virus-specific materials were also present. One of these was probably neuraminidase and the other the specific serum blocking substance.

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