Skip to main content
The Journal of Hygiene logoLink to The Journal of Hygiene
. 1970 Jun;68(2):273–282. doi: 10.1017/s0022172400028734

An adhesive surface sampling technique for airborne viruses

G Thomas
PMCID: PMC2130810  PMID: 4317107

Abstract

A mixture of sucrose, glycerol and bovine serum albumin produces a stable coating in a Petri dish which remains adhesive for up to an hour when exposed in a slit sampler. Virus aerosols can be collected on this surface followed by the direct addition of cell cultures to demonstrate the presence of viable virus. The technique is applicable to the Andersen sampler. A modified version of this sampler has been produced with the same particle collection efficiency as the standard Andersen sampler. The plaque counts obtained by the adhesive surface sampling technique are believed to give an indication of the number of particles collected bearing viable virus.

Full text

PDF

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ANDERSEN A. A. New sampler for the collection, sizing, and enumeration of viable airborne particles. J Bacteriol. 1958 Nov;76(5):471–484. doi: 10.1128/jb.76.5.471-484.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. APPLEYARD G., WESTWOOD J. C. THE GROWTH OF RABBITPOX VIRUS IN TISSUE CULTURE. J Gen Microbiol. 1964 Dec;37:391–401. doi: 10.1099/00221287-37-3-391. [DOI] [PubMed] [Google Scholar]
  3. ARTENSTEIN M. S., CADIGAN F. C., Jr AIR SAMPLING IN VIRAL RESPIRATORY DISEASE. Arch Environ Health. 1964 Jul;9:58–60. doi: 10.1080/00039896.1964.10663793. [DOI] [PubMed] [Google Scholar]
  4. An Approach to the Kinetics of Microbiological Deterioration. Appl Microbiol. 1961 Mar;9(2):105–105. doi: 10.1128/am.9.2.105-105.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Artenstein M. S., Miller W. S., Lamson T. H., Brandt B. L. Large-volume air sampling for Meningococci and Adenoviruses. Am J Epidemiol. 1968 May;87(3):567–577. doi: 10.1093/oxfordjournals.aje.a120847. [DOI] [PubMed] [Google Scholar]
  6. COUCH R. B., GERONE P. J., CATE T. R., GRIFFITH W. R., ALLING D. W., KNIGHT V. PREPARATION AND PROPERTIES OF A SMALL-PARTICLE AEROSOL OF COXSACKIE A21. Proc Soc Exp Biol Med. 1965 Mar;118:818–822. doi: 10.3181/00379727-118-29981. [DOI] [PubMed] [Google Scholar]
  7. DAHLGREN C. M., DECKER H. M., HARSTAD J. B. A slit sampler for collecting T-3 bacteriophage and Venezuelan equine encephalomvelitis virus. I. Studies with T-3 bacteriophage. Appl Microbiol. 1961 Mar;9:103–105. doi: 10.1128/am.9.2.103-105.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Ehrlich R., Miller S., Idoine L. S. Evaluation of slit sampler in quantitative studies of bacterial aerosols. Appl Microbiol. 1966 May;14(3):328–330. doi: 10.1128/am.14.3.328-330.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. GOLDBERG L. J., WATKINS H. M., BOERKE E. E., CHATIGNY M. A. The use of a rotating drum for the study of aerosols over extended periods of time. Am J Hyg. 1958 Jul;68(1):85–93. doi: 10.1093/oxfordjournals.aje.a119954. [DOI] [PubMed] [Google Scholar]
  10. Gerone P. J., Couch R. B., Keefer G. V., Douglas R. G., Derrenbacher E. B., Knight V. Assessment of experimental and natural viral aerosols. Bacteriol Rev. 1966 Sep;30(3):576–588. doi: 10.1128/br.30.3.576-588.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. HENDERSON D. W. An apparatus for the study of airborne infection. J Hyg (Lond) 1952 Mar;50(1):53–68. doi: 10.1017/s0022172400019422. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. JENSEN M. M. INACTIVATION OF AIRBORNE VIRUSES BY ULTRAVIOLET IRRADIATION. Appl Microbiol. 1964 Sep;12:418–420. doi: 10.1128/am.12.5.418-420.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. May K. R. Multistage liquid impinger. Bacteriol Rev. 1966 Sep;30(3):559–570. doi: 10.1128/br.30.3.559-570.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. McLean D. M., Bannatyne R. M., Givan K. F. Myxovirus dissemination by air. Can Med Assoc J. 1967 Jun 3;96(22):1449–1453. [PMC free article] [PubMed] [Google Scholar]
  15. Westwood J. C., Boulter E. A., Bowen E. T., Maber H. B. Experimental respiratory infection with poxviruses. I. Clinical virological and epidemiological studies. Br J Exp Pathol. 1966 Oct;47(5):453–465. [PMC free article] [PubMed] [Google Scholar]
  16. Winkler W. G. Airborne rabies virus isolation. Wildl Dis. 1968 Apr;4(2):37–40. doi: 10.7589/0090-3558-4.2.37. [DOI] [PubMed] [Google Scholar]
  17. Wolfe L. G., Griesemer R. A., Farrell R. L. Experimental aerosol transmission of Yaba virus in monkeys. J Natl Cancer Inst. 1968 Nov;41(5):1175–1195. [PubMed] [Google Scholar]

Articles from The Journal of Hygiene are provided here courtesy of Cambridge University Press

RESOURCES