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. 1990 Sep;172(9):5135–5139. doi: 10.1128/jb.172.9.5135-5139.1990

Phosphorylated tyrosine in the flagellum filament protein of Pseudomonas aeruginosa.

K Kelly-Wintenberg 1, T Anderson 1, T C Montie 1
PMCID: PMC213172  PMID: 1697577

Abstract

Purified flagella from two strains of 32P-labeled Pseudomonas aeruginosa were shown to be phosphorylated. This was confirmed by autoradiography of flagellin protein in polyacrylamide gels. Thin-layer electrophoresis and autoradiography of flagellin partial hydrolysates indicated that phosphotyrosine was the major phosphorylated amino acid. High-pressure liquid chromatographic analysis confirmed the presence of phosphotyrosine in flagellum filament protein. Preliminary data indicated that less than one tyrosine per subunit was phosphorylated. No evidence was found for phosphorylation of serine or threonine. A function related to tyrosine phosphorylation has not been determined.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bell A., Hancock R. E. Outer membrane protein H1 of Pseudomonas aeruginosa: purification of the protein and cloning and nucleotide sequence of the gene. J Bacteriol. 1989 Jun;171(6):3211–3217. doi: 10.1128/jb.171.6.3211-3217.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Cooper J. A., Sefton B. M., Hunter T. Detection and quantification of phosphotyrosine in proteins. Methods Enzymol. 1983;99:387–402. doi: 10.1016/0076-6879(83)99075-4. [DOI] [PubMed] [Google Scholar]
  3. Cozzone A. J. Protein phosphorylation in prokaryotes. Annu Rev Microbiol. 1988;42:97–125. doi: 10.1146/annurev.mi.42.100188.000525. [DOI] [PubMed] [Google Scholar]
  4. Craven R., Montie T. C. Regulation of Pseudomonas aeruginosa chemotaxis by the nitrogen source. J Bacteriol. 1985 Nov;164(2):544–549. doi: 10.1128/jb.164.2.544-549.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Drake D., Montie T. C. Flagella, motility and invasive virulence of Pseudomonas aeruginosa. J Gen Microbiol. 1988 Jan;134(1):43–52. doi: 10.1099/00221287-134-1-43. [DOI] [PubMed] [Google Scholar]
  6. Drake D., Montie T. C. Protection against Pseudomonas aeruginosa infection by passive transfer of anti-flagellar serum. Can J Microbiol. 1987 Sep;33(9):755–763. doi: 10.1139/m87-130. [DOI] [PubMed] [Google Scholar]
  7. Foster R., Thorner J., Martin G. S. Nucleotidylation, not phosphorylation, is the major source of the phosphotyrosine detected in enteric bacteria. J Bacteriol. 1989 Jan;171(1):272–279. doi: 10.1128/jb.171.1.272-279.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hindahl M. S., Frank D. W., Iglewski B. H. Molecular studies of a positive regulator of toxin A synthesis in Pseudomonas aeruginosa. Antibiot Chemother (1971) 1987;39:279–289. doi: 10.1159/000414353. [DOI] [PubMed] [Google Scholar]
  9. Kelly-Wintenberg K., Montie T. C. Cloning and expression of Pseudomonas aeruginosa flagellin in Escherichia coli. J Bacteriol. 1989 Nov;171(11):6357–6362. doi: 10.1128/jb.171.11.6357-6362.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Logan S. M., Trust T. J., Guerry P. Evidence for posttranslational modification and gene duplication of Campylobacter flagellin. J Bacteriol. 1989 Jun;171(6):3031–3038. doi: 10.1128/jb.171.6.3031-3038.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Montie T. C., Doyle-Huntzinger D., Craven R. C., Holder I. A. Loss of virulence associated with absence of flagellum in an isogenic mutant of Pseudomonas aeruginosa in the burned-mouse model. Infect Immun. 1982 Dec;38(3):1296–1298. doi: 10.1128/iai.38.3.1296-1298.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Montie T. C., Drake D., Sellin H., Slater O., Edmonds S. Motility, virulence, and protection with a flagella vaccine against Pseudomonas aeruginosa infection. Antibiot Chemother (1971) 1987;39:233–248. doi: 10.1159/000414349. [DOI] [PubMed] [Google Scholar]
  13. Pairoba C. F., Vallejos R. H. Protein phosphorylation in purple photosynthetic bacteria. Biochimie. 1989 Sep-Oct;71(9-10):1039–1041. doi: 10.1016/0300-9084(89)90108-9. [DOI] [PubMed] [Google Scholar]
  14. Totten P. A., Lara J. C., Lory S. The rpoN gene product of Pseudomonas aeruginosa is required for expression of diverse genes, including the flagellin gene. J Bacteriol. 1990 Jan;172(1):389–396. doi: 10.1128/jb.172.1.389-396.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Vasil M. L., Ogle J. W., Grant C. C., Vasil A. I. Recombinant DNA approaches to the study of the regulation of virulence factors and epidemiology of Pseudomonas aeruginosa. Antibiot Chemother (1971) 1987;39:264–278. doi: 10.1159/000414352. [DOI] [PubMed] [Google Scholar]

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