Abstract
The substance in syphilitic serum which is responsible for the Wassermann reaction, like that which determines the diagnostic flocculation tests, is associated with the globulin fraction of serum. Every positive Wassermann is accompanied by microscopic (or submicroscopic) aggregation, which is not an essential feature of the reaction; conversely, after every positive flocculation test, the washed precipitate will fix complement. An excess of antigen removes both flocculating and complement-fixing substances completely (>95 per cent). Heating the lipoid-reagin precipitate to 100° for 1 minute destroys the sensitizing film of reagin globulin; the avidity for complement disappears simultaneously. Both the flocculating and complement-fixing properties of syphilitic serum are therefore determined by the same substance, a specifically altered fraction of the serum globulin, reagin. The Wassermann reaction is thus entirely analogous to complement fixation by any antigen-antibody complex. The same film of denatured serum globulin which sensitizes the antigen particles, whether red cells, bacteria, protein, or colloidal lipoid particles, to discharge and aggregation by electrolytes, also endows them with an avidity for complement. The pathogenesis of reagin will be discussed in a forthcoming paper.
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Selected References
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- Eagle H. STUDIES IN THE SEROLOGY OF SYPHILIS : I. THE MECHANISM OF THE FLOCCULATION REACTIONS. J Exp Med. 1930 Oct 31;52(5):717–738. doi: 10.1084/jem.52.5.717. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mudd S., Mudd E. B. ON THE MECHANISM OF THE SERUM SENSITIZATION OF ACID-FAST BACTERIA. J Exp Med. 1927 Jun 30;46(1):173–195. doi: 10.1084/jem.46.1.173. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mudd S., Mudd E. B. ON THE SURFACE COMPOSITION OF NORMAL AND SENSITIZED MAMMALIAN BLOOD CELLS. J Exp Med. 1926 Jan 1;43(1):127–142. doi: 10.1084/jem.43.1.127. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shibley G. S. STUDIES IN AGGLUTINATION : III. ON THE MECHANISM OF THE AGGLUTINATION OF BACTERIA BY SPECIFIC AGGLUTINATING SERUM. J Exp Med. 1926 Oct 31;44(5):667–681. doi: 10.1084/jem.44.5.667. [DOI] [PMC free article] [PubMed] [Google Scholar]