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. 1932 Mar 31;55(4):555–561. doi: 10.1084/jem.55.4.555

QUANTITATIVE STUDIES ON THE PRECIPITIN REACTION

THE DETERMINATION OF SMALL AMOUNTS OF A SPECIFIC POLYSACCHARIDE

Michael Heidelberger 1, Forrest E Kendall 1
PMCID: PMC2132119  PMID: 19870012

Abstract

A method, based on the precipitin reaction, is given for the micro determination of the specific polysaccharide of Type III pneumococcus. As little as 0.01 mg. of S III can be determined. The method should be applicable to any specific polysaccharide upon standardization of an homologous antibody solution or antiserum in the region of excess antibody.

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Selected References

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  1. Heidelberger M., Kendall F. E. A QUANTITATIVE STUDY OF THE PRECIPITIN REACTION BETWEEN TYPE III PNEUMOCOCCUS POLYSACCHARIDE AND PURIFIED HOMOLOGOUS ANTIBODY. J Exp Med. 1929 Nov 30;50(6):809–823. doi: 10.1084/jem.50.6.809. [DOI] [PMC free article] [PubMed] [Google Scholar]
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