Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1990 Sep;172(9):5503–5505. doi: 10.1128/jb.172.9.5503-5505.1990

SOS repair can be about as effective for single-stranded DNA as for double-stranded DNA and even more so.

I Tessman 1
PMCID: PMC213222  PMID: 2144278

Abstract

As ordinarily measured, the SOS repair of damaged DNA by Weigle reactivation appears to be more effective for double-stranded (ds) than for single-stranded (ss) DNA bacteriophages. A complicating feature, which is usually not considered, is the possibility of DNA-protein cross-linking of ssDNA to the viral capsid, which would conceivably be an extraneous source of nonreactivable lesions. This idea is supported in studies of phage S13 by the observation that photoreactivation more than doubles when naked ssDNA is substituted for encapsidated ssDNA as the UV target. The same effect was observed for Weigle reactivation; there was little, if any, difference in the reactivation of ssDNA and dsDNA when naked DNA was irradiated. Moreover, in a uvrA mutant, ssDNA actually had the advantage; Weigle reactivation was then more than twice as effective for ssDNA as for dsDNA. It is also shown that when a suitable measure of Weigle mutagenesis is used, there is no convincing evidence that dsDNA is mutagenized more effectively than ssDNA.

Full text

PDF
5503

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Calsou P., Defais M. Weigle reactivation and mutagenesis of bacteriophage lambda in lexA(Def) mutants of E. coli K12. Mol Gen Genet. 1985;201(2):329–333. doi: 10.1007/BF00425679. [DOI] [PubMed] [Google Scholar]
  2. Cooper P. K. Characterization of long patch excision repair of DNA in ultraviolet-irradiated Escherichia coli: an inducible function under rec-lex control. Mol Gen Genet. 1982;185(2):189–197. doi: 10.1007/BF00330785. [DOI] [PubMed] [Google Scholar]
  3. DAVID C. N. UV INACTIVATION AND THYMINE DIMERIZATION IN BACTERIOPHAGE PHI X. Z Vererbungsl. 1964 Dec 30;95:318–325. doi: 10.1007/BF01268664. [DOI] [PubMed] [Google Scholar]
  4. Defais M., Lesca C., Monsarrat B., Hanawalt P. Translesion synthesis is the main component of SOS repair in bacteriophage lambda DNA. J Bacteriol. 1989 Sep;171(9):4938–4944. doi: 10.1128/jb.171.9.4938-4944.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Defais M. Role of the E. coli umuC gene product in the repair of single-stranded DNA phage. Mol Gen Genet. 1983;192(3):509–511. doi: 10.1007/BF00392198. [DOI] [PubMed] [Google Scholar]
  6. Elledge S. J., Walker G. C. Proteins required for ultraviolet light and chemical mutagenesis. Identification of the products of the umuC locus of Escherichia coli. J Mol Biol. 1983 Feb 25;164(2):175–192. doi: 10.1016/0022-2836(83)90074-8. [DOI] [PubMed] [Google Scholar]
  7. TESSMAN E. S., OZAKI T. The interaction of phage S13 with ultraviolet-irradiated host cells and properties of the ultraviolet-irradiated phage. Virology. 1960 Nov;12:431–449. doi: 10.1016/0042-6822(60)90165-3. [DOI] [PubMed] [Google Scholar]
  8. Tessman E. S. Mutants of bacteriophage S13 blocked in infectious DNA synthesis. J Mol Biol. 1966 May;17(1):218–236. doi: 10.1016/s0022-2836(66)80104-3. [DOI] [PubMed] [Google Scholar]
  9. Tessman E. S., Peterson P. Plaque color method for rapid isolation of novel recA mutants of Escherichia coli K-12: new classes of protease-constitutive recA mutants. J Bacteriol. 1985 Aug;163(2):677–687. doi: 10.1128/jb.163.2.677-687.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Tessman E. S., Tessman I., Peterson P. K., Forestal J. D. Roles of RecA protease and recombinase activities of Escherichia coli in spontaneous and UV-induced mutagenesis and in Weigle repair. J Bacteriol. 1986 Dec;168(3):1159–1164. doi: 10.1128/jb.168.3.1159-1164.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Tessman I., Morrison H., Bernasconi C., Pandey G., Ekanayake L. Photochemical inactivation of single-stranded viral DNA in the presence of urocanic acid. Photochem Photobiol. 1983 Jul;38(1):29–35. doi: 10.1111/j.1751-1097.1983.tb08362.x. [DOI] [PubMed] [Google Scholar]
  12. Tessman I. UV-induced mutagenesis of phage S13 can occur in the absence of the RecA and UmuC proteins of Escherichia coli. Proc Natl Acad Sci U S A. 1985 Oct;82(19):6614–6618. doi: 10.1073/pnas.82.19.6614. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Walker G. C., Dobson P. P. Mutagenesis and repair deficiencies of Escherichia coli umuC mutants are suppressed by the plasmid pKM101. Mol Gen Genet. 1979 Apr 17;172(1):17–24. doi: 10.1007/BF00276210. [DOI] [PubMed] [Google Scholar]
  14. Weigle J. J. Induction of Mutations in a Bacterial Virus. Proc Natl Acad Sci U S A. 1953 Jul;39(7):628–636. doi: 10.1073/pnas.39.7.628. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES