Abstract
The relationship between the experimental measurement of the cross-linking of bacterial peptidoglycan and the mode of its insertion is analyzed. The cross-linking value, in practice and in theory, is independent of the pattern of strand insertion. Since the measure of the mode or pattern of insertion is the acceptor/donor radioactivity ratio (ADRR), no correction need be made for the ADRR. The measurement of cross-linking using radioactivity is independent of the labeling time, the specific activity of the label, and the mode of strand insertion. It is not concluded, however, that cross-linking does increase during the division cycle.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Burman L. G., Park J. T. Changes in the composition of Escherichia coli murein as it ages during exponential growth. J Bacteriol. 1983 Aug;155(2):447–453. doi: 10.1128/jb.155.2.447-453.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Burman L. G., Park J. T. Molecular model for elongation of the murein sacculus of Escherichia coli. Proc Natl Acad Sci U S A. 1984 Mar;81(6):1844–1848. doi: 10.1073/pnas.81.6.1844. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cooper S., Hsieh M. L., Guenther B. Mode of peptidoglycan synthesis in Salmonella typhimurium: single-strand insertion. J Bacteriol. 1988 Aug;170(8):3509–3512. doi: 10.1128/jb.170.8.3509-3512.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Glauner B., Höltje J. V., Schwarz U. The composition of the murein of Escherichia coli. J Biol Chem. 1988 Jul 25;263(21):10088–10095. [PubMed] [Google Scholar]
- Goodell E. W., Schwarz U. Cleavage and resynthesis of peptide cross bridges in Escherichia coli murein. J Bacteriol. 1983 Oct;156(1):136–140. doi: 10.1128/jb.156.1.136-140.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Olijhoek A. J., Klencke S., Pas E., Nanninga N., Schwarz U. Volume growth, murein synthesis, and murein cross-linkage during the division cycle of Escherichia coli PA3092. J Bacteriol. 1982 Dec;152(3):1248–1254. doi: 10.1128/jb.152.3.1248-1254.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- de Jonge B. L., Wientjes F. B., Jurida I., Driehuis F., Wouters J. T., Nanninga N. Peptidoglycan synthesis during the cell cycle of Escherichia coli: composition and mode of insertion. J Bacteriol. 1989 Nov;171(11):5783–5794. doi: 10.1128/jb.171.11.5783-5794.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
- de Pedro M. A., Schwarz U. Heterogeneity of newly inserted and preexisting murein in the sacculus of Escherichia coli. Proc Natl Acad Sci U S A. 1981 Sep;78(9):5856–5860. doi: 10.1073/pnas.78.9.5856. [DOI] [PMC free article] [PubMed] [Google Scholar]