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. 1933 Jul 31;58(2):211–226. doi: 10.1084/jem.58.2.211

THE TITRATION OF YELLOW FEVER VIRUS IN STEGOMYIA MOSQUITOES

Nelson C Davis 1, Martin Frobisher Jr 1, Wray Lloyd 1
PMCID: PMC2132287  PMID: 19870190

Abstract

Titrations were made of yellow fever virus in stegomyia mosquitoes, using rhesus monkeys as test animals. It was found that: (a) The average mosquito immediately after engorging on highly infectious blood contained between 1 and 2 million lethal doses of virus. The titer of freshly ingested blood was as high as 1 billion lethal doses of virus per cubic centimeter. (b) During the fortnight succeeding a meal on infectious blood there occurred a reduction of titratable virus to not more than 1 per cent of that present in the freshly fed insects. (c) The titer was somewhat higher at later periods. This rise in titer signified possibly not a multiplication, but merely an increase of extracellular virus and of that easily freed by grinding to a titratable form. (d) At no later stage did the quantity of titratable virus equal that demonstrable in freshly fed insects.

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Selected References

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  1. Huff C. G. A PROPOSED CLASSIFICATION OF DISEASE TRANSMISSIONS BY ARTHROPODS. Science. 1931 Nov 6;74(1923):456–457. doi: 10.1126/science.74.1923.456-a. [DOI] [PubMed] [Google Scholar]

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