Abstract
1. The preparation is described of a deep red protein dye, R-salt-azo-benzidine-azo-crystalline egg albumen, which contains no more than traces of protein with the original egg albumen specificity. 2. Based on previous publications of the writers, a quantitative method is given for the micro estimation of precipitin in the antisera to the dye. The method gives the actual weight of precipitin and may be applied to the determination of the maximum amount of precipitable antibody in any antiserum. 3. Data are given (1) on the influence of the period between the final injection and the bleeding on the precipitin content of rabbit antisera to the azo protein; (2) on the magnitude of the antibody response following the injection of multiple doses of the antigen varying within wide limits; (3) on the variations in the precipitin content of the sera of rabbits given successive courses of antigen injections; and (4) on the stability of antisera stored in the cold. 4. Four antisera were obtained in which over 100 times as much precipitin was recovered as the amount of antigen injected. This supplements the growing mass of evidence against the theory that specific antigen fragments are actually incorporated into the antibody molecule.
Full Text
The Full Text of this article is available as a PDF (813.8 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Goebel W. F., Avery O. T. CHEMO-IMMUNOLOGICAL STUDIES ON CONJUGATED CARBOHYDRATE-PROTEINS : IV. THE SYNTHESIS OF THEp-AMINOBENZYL ETHER OF THE SOLUBLE SPECIFIC SUBSTANCE OF TYPE III PNEUMOCOCCUS AND ITS COUPLING WITH PROTEIN. J Exp Med. 1931 Jul 31;54(3):431–436. doi: 10.1084/jem.54.3.431. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Heidelberger M., Kendall F. E. A QUANTITATIVE STUDY OF THE PRECIPITIN REACTION BETWEEN TYPE III PNEUMOCOCCUS POLYSACCHARIDE AND PURIFIED HOMOLOGOUS ANTIBODY. J Exp Med. 1929 Nov 30;50(6):809–823. doi: 10.1084/jem.50.6.809. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Heidelberger M., Kendall F. E. QUANTITATIVE STUDIES ON THE PRECIPITIN REACTION : THE DETERMINATION OF SMALL AMOUNTS OF A SPECIFIC POLYSACCHARIDE. J Exp Med. 1932 Mar 31;55(4):555–561. doi: 10.1084/jem.55.4.555. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Heidelberger M., Kendall F. E. SPECIAL ARTICLES. Science. 1930 Sep 5;72(1862):252–253. doi: 10.1126/science.72.1862.252. [DOI] [PubMed] [Google Scholar]
- Heidelberger M., Sia R. H., Kendall F. E. SPECIFIC PRECIPITATION AND MOUSE PROTECTION IN TYPE I ANTIPNEUMOCOCCUS SERA. J Exp Med. 1930 Sep 30;52(4):477–483. doi: 10.1084/jem.52.4.477. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Landsteiner K., van der Scheer J. SEROLOGICAL REACTIONS WITH SIMPLE CHEMICAL COMPOUNDS (PRECIPITIN REACTIONS). J Exp Med. 1932 Aug 31;56(3):399–409. doi: 10.1084/jem.56.3.399. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Smithburn K. C., Sabin F. R. THE CELLULAR REACTIONS TO LIPOID FRACTIONS FROM ACID-FAST BACILLI. J Exp Med. 1932 Nov 30;56(6):867–891. doi: 10.1084/jem.56.6.867. [DOI] [PMC free article] [PubMed] [Google Scholar]