Abstract
Methods of preparation and certain properties of the "nucleoproteins" of the following organisms are described: gonococcus, meningococcus, Micrococcus catarrhalis, R pneumococcus, Streptococcus hemolyticus, Staphylococcus aureus. No essential differences between the nucleoproteins and the intact cells of gonococcus and meningococcus were observed in their ability to engender immune substances (precipitins), to induce bacterial allergy in rabbits, or to elicit cutaneous reactions (of the delayed type) in rabbits rendered hypersensitive to these organisms. Measured by their lethal action in mice, the toxicity of gonococcal and meningococcal nucleoproteins was found to be but slightly less than that of the intact cells. It seems probable, therefore, that the toxic action of these organisms is due, chiefly or entirely, to some constituent of the nucleoprotein fraction. Extraction with acetone and ether in the cold did not reduce appreciably the toxicity of these organisms and their nucleoproteins, nor alter their immunological behavior. Cross-precipitin reactions suggested that gonococcal nucleoprotein contains an antigenic factor in common with the non-encapsulated pneumococcus cell, and meningococcal nucleoprotein one in common with the capsular material of Pneumococcus Type III. Tryptic digestion destroys these antigenic factors, but not those responsible for the cross-reactions within the genus Neisseria.
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Selected References
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- Avery O. T., Heidelberger M. IMMUNOLOGICAL RELATIONSHIPS OF CELL CONSTITUENTS OF PNEUMOCOCCUS. J Exp Med. 1923 Jun 30;38(1):81–85. doi: 10.1084/jem.38.1.81. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Heidelberger M., Avery O. T. THE SOLUBLE SPECIFIC SUBSTANCE OF PNEUMOCOCCUS. J Exp Med. 1923 Jun 30;38(1):73–79. doi: 10.1084/jem.38.1.73. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Heidelberger M., Kendall F. E. PROTEIN FRACTIONS OF A SCARLATINAL STRAIN OF STREPTOCOCCUS HEMOLYTICUS. J Exp Med. 1931 Sep 30;54(4):515–531. doi: 10.1084/jem.54.4.515. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lancefield R. C. THE IMMUNOLOGICAL RELATIONSHIPS OF STREPTOCOCCUS VIRIDANS AND CERTAIN OF ITS CHEMICAL FRACTIONS : II. SEROLOGICAL REACTIONS OBTAINED WITH ANTINUCLEOPROTEIN SERA. J Exp Med. 1925 Aug 31;42(3):397–412. doi: 10.1084/jem.42.3.397. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Miller C. P., Boor A. K. THE CARBOHYDRATES OF GONOCOCCUS AND MENINGOCOCCUS : I. THE ALCOHOL-PRECIPITABLE FRACTION. J Exp Med. 1934 Jan 1;59(1):75–82. doi: 10.1084/jem.59.1.75. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Miller C. P., Castles R. SOME OBSERVATIONS ON THE SPECIFICITY OF BACTERIAL ALLERGY TO CERTAIN OF THE NEISSERIAE. J Exp Med. 1933 Sep 30;58(4):435–450. doi: 10.1084/jem.58.4.435. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Miller C. P., Hastings A. B., Castles R. The Influence of Inorganic Salts on the Multiplication of Gonococcus. J Bacteriol. 1932 Dec;24(6):439–455. doi: 10.1128/jb.24.6.439-455.1932. [DOI] [PMC free article] [PubMed] [Google Scholar]