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. 1934 Oct 31;60(5):599–617. doi: 10.1084/jem.60.5.599

CHEMO-IMMUNOLOGICAL STUDIES ON CONJUGATED CARBOHYDRATE-PROTEINS

IX. THE SPECIFICITY OF ANTIGENS PREPARED BY COMBINING THEp-AMINOPHENOL GLYCOSIDES OF DISACCHARIDES WITH PROTEIN

Walther F Goebel 1, Oswald T Avery 1, Frank H Babers 1
PMCID: PMC2132428  PMID: 19870325

Abstract

The results of the present study indicate that by means of serological reactions it is possible to differentiate selectively the p-aminophenol glycosides of maltose, cellobiose, gentiobiose, and lactose. The immunological specificity of disaccharide-protein antigens prepared from these derivatives, irrespective of the nature of the conjoined protein, is determined by (1) the glycoside molecule as a whole, (2) the configuration of the terminal hexose molecule, and (3) the position of linkage of the two hexose units in the carbohydrate radical. The specificity of the antibodies induced by the disaccharide antigens appears to be more sharply defined when the configuration of the terminal hexose is of the β rather than of the α type.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Avery O. T., Goebel W. F., Babers F. H. CHEMO-IMMUNOLOGICAL STUDIES ON CONJUGATED CARBOHYDRATE-PROTEINS : VII. IMMUNOLOGICAL SPECIFICITY OF ANTIGENS PREPARED BY COMBININGalpha- AND beta-GLUCOSIDES OF GLUCOSE WITH PROTEINS. J Exp Med. 1932 Apr 30;55(5):769–780. doi: 10.1084/jem.55.5.769. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Avery O. T., Goebel W. F. CHEMO-IMMUNOLOGICAL STUDIES ON CONJUGATED CARBOHYDRATE-PROTEINS : II. IMMUNOLOGICAL SPECIFICITY OF SYNTHETIC SUGAR-PROTEIN ANTIGENS. J Exp Med. 1929 Sep 30;50(4):533–550. doi: 10.1084/jem.50.4.533. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Goebel W. F., Babers F. H., Avery O. T. CHEMO-IMMUNOLOGICAL STUDIES ON CONJUGATED CARBOHYDRATE-PROTEINS : VI. THE SYNTHESIS OFp-AMINOPHENOLalpha-GLUCOSIDE AND ITS COUPLING WITH PROTEIN. J Exp Med. 1932 Apr 30;55(5):761–767. doi: 10.1084/jem.55.5.761. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Heidelberger M., Kendall F. E. QUANTITATIVE STUDIES ON THE PRECIPITIN REACTION : THE ROLE OF MULTIPLE REACTIVE GROUPS IN ANTIGEN-ANTIBODY UNION AS ILLUSTRATED BY AN INSTANCE OF CROSS-PRECIPITATION. J Exp Med. 1934 Mar 31;59(4):519–528. doi: 10.1084/jem.59.4.519. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Landsteiner K., van der Scheer J. ON THE SEROLOGICAL SPECIFICITY OF PEPTIDES. II. J Exp Med. 1934 May 31;59(6):769–780. doi: 10.1084/jem.59.6.769. [DOI] [PMC free article] [PubMed] [Google Scholar]

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