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. 1990 Aug;172(8):4315–4321. doi: 10.1128/jb.172.8.4315-4321.1990

Cloning of aldB, which encodes alpha-acetolactate decarboxylase, an exoenzyme from Bacillus brevis.

B Diderichsen 1, U Wedsted 1, L Hedegaard 1, B R Jensen 1, C Sjøholm 1
PMCID: PMC213256  PMID: 2198252

Abstract

A gene for alpha-acetolactate decarboxylase (ALDC) was cloned from Bacillus brevis in Escherichia coli and in Bacillus subtilis. The 1.3-kilobase-pair nucleotide sequence of the gene, aldB, encoding ALDC and its flanking regions was determined. An open reading frame of 285 amino acids included a typical N-terminal signal peptide of 24 or 27 amino acids. A B. subtilis strain harboring the aldB gene on a recombinant plasmid processed and secreted ALDC. In contrast, a similar enzyme from Enterobacter aerogenes is intracellular.

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Selected References

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