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. 1990 Aug;172(8):4728–4731. doi: 10.1128/jb.172.8.4728-4731.1990

Identification of a locus that regulates multiple functions in Pseudomonas solanacearum.

Y Huang 1, L Sequeira 1
PMCID: PMC213316  PMID: 2165483

Abstract

When Pseudomonas solanacearum K60 carries a multicopy plasmid containing cosmid clone pE6C (from the wild-type strain K60) or pBE6 (from the nonpathogenic strain B1), several phenotypic changes are observed, including the following: loss of virulence, reduced extracellular polysaccharide production, and increased polygalacturonase activity. Both cosmids contain a common 8-kilobase DNA region that is required for the phenotype shift. Saturation mutagenesis of pBE6 with Tn3-gus suggested that a single transcriptional unit of at least 1 kilobase is responsible for the phenotype shift. In maxicell assays, subclones containing this transcriptional unit expressed a single protein of about 25 kilodaltons.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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