Abstract
1. Anantiserum which specifically protects mice against a virulent culture (M variant) of the hemolytic streptococcus contains specific opsonin. Phagocytosis of the organisms can be observed in the peritoneum of the protected mouse. 2. An antiserum prepared by injecting an animal with the living M variant specifically opsonizes both the F and the M variant of the strain. 3. Evidence is presented which indicates the probable identity of the specific opsonin and the anti-M precipitin of Lancefield (7). Agglutination appears to be dependent upon a different antibody. 4. It is possible to type the hemolytic streptococci by means of specific opsonins, and the opsonic method has certain advantages over agglutination, precipitation, and mouse protection tests. It is evident from what little has been done that there are many types. 5. The serum of infants contains no opsonin for the virulent hemolytic streptococci, but the serum of adults may contain specific opsonins for certain strains. Inasmuch as no opsonins were demonstrable in two polyvalent antibacterial sera examined, the possibilities of therapeutic transfusion are discussed.
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Selected References
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- Dochez A. R., Avery O. T., Lancefield R. C. STUDIES ON THE BIOLOGY OF STREPTOCOCCUS : I. ANTIGENIC RELATIONSHIPS BETWEEN STRAINS OF STREPTOCOCCUS HAEMOLYTICUS. J Exp Med. 1919 Sep 1;30(3):179–213. doi: 10.1084/jem.30.3.179. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lancefield R. C., Todd E. W. ANTIGENIC DIFFERENCES BETWEEN MATT HEMOLYTIC STREPTOCOCCI AND THEIR GLOSSY VARIANTS. J Exp Med. 1928 Nov 30;48(6):769–790. doi: 10.1084/jem.48.6.769. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Menkin V. STUDIES ON INFLAMMATION : IX. A FACTOR IN THE MECHANISM OF INVASIVENESS BY PYOGENIC BACTERIA. J Exp Med. 1933 May 31;57(6):977–991. doi: 10.1084/jem.57.6.977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Seastone C. V. Capsules in Young Cultures of Streptococcus hemolyticus. J Bacteriol. 1934 Nov;28(5):481–487. doi: 10.1128/jb.28.5.481-487.1934. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tillett W. S., Garner R. L. THE FIBRINOLYTIC ACTIVITY OF HEMOLYTIC STREPTOCOCCI. J Exp Med. 1933 Sep 30;58(4):485–502. doi: 10.1084/jem.58.4.485. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ward H. K., Enders J. F. AN ANALYSIS OF THE OPSONIC AND TROPIC ACTION OF NORMAL AND IMMUNE SERA BASED ON EXPERIMENTS WITH THE PNEUMOCOCCUS. J Exp Med. 1933 Mar 31;57(4):527–547. doi: 10.1084/jem.57.4.527. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ward H. K. OBSERVATIONS ON THE PHAGOCYTOSIS OF THE PNEUMOCOCCUS BY HUMAN WHOLE BLOOD : I. THE NORMAL PHAGOCYTIC TITRE, AND THE ANTI-PHAGOCYTIC EFFECT OF THE SPECIFIC SOLUBLE SUBSTANCE. J Exp Med. 1930 Apr 30;51(5):675–684. doi: 10.1084/jem.51.5.675. [DOI] [PMC free article] [PubMed] [Google Scholar]