Abstract
1. The thermolability of the specific polysaccharides of Types I, II, and III pneumococcus has been shown by three independent methods: (a) diminution of the viscosity of solutions on heating; (b) decrease in the amount of antibody precipitated from homologous rabbit antisera; and (c) increased tendency (S III) to pass through a collodion membrane. 2. These effects may be explained most simply as a partial depolymerization under the influence of heat. In air, particularly in the presence of broth, oxidation also appears to be involved. 3. Improved and simpler methods of preparation based on these findings, are given for S I, S II, and S III. The resulting products precipitate more anti-S from homologous rabbit antisera than do the earlier preparations. 4. The methyl glycoside of methyl galacturonate has been isolated from the hydrolytic products of S I, and evidence of the ultimate structural unit obtained.
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Selected References
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- Avery O. T., Goebel W. F. CHEMOIMMUNOLOGICAL STUDIES ON THE SOLUBLE SPECIFIC SUBSTANCE OF PNEUMOCOCCUS : I. THE ISOLATION AND PROPERTIES OF THE ACETYL POLYSACCHARIDE OF PNEUMOCOCCUS TYPE I. J Exp Med. 1933 Nov 30;58(6):731–755. doi: 10.1084/jem.58.6.731. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dubos R. THE BACTERIOSTATIC ACTION OF CERTAIN COMPONENTS OF COMMERCIAL PEPTONES AS AFFECTED BY CONDITIONS OF OXIDATION AND REDUCTION. J Exp Med. 1930 Aug 31;52(3):331–345. doi: 10.1084/jem.52.3.331. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Heidelberger M., Goebel W. F., Avery O. T. THE SOLUBLE SPECIFIC SUBSTANCE OF PNEUMOCOCCUS : THIRD PAPER. J Exp Med. 1925 Oct 31;42(5):727–745. doi: 10.1084/jem.42.5.727. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Heidelberger M., Kabat E. A. CHEMICAL STUDIES ON BACTERIAL AGGLUTINATION : I. A METHOD. J Exp Med. 1934 Oct 31;60(5):643–653. doi: 10.1084/jem.60.5.643. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Heidelberger M., Kendall F. E. QUANTITATIVE STUDIES ON THE PRECIPITIN REACTION : THE DETERMINATION OF SMALL AMOUNTS OF A SPECIFIC POLYSACCHARIDE. J Exp Med. 1932 Mar 31;55(4):555–561. doi: 10.1084/jem.55.4.555. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Heidelberger M., Kendall F. E. SPECIFIC AND NON-SPECIFIC POLYSACCHARIDES OF TYPE IV PNEUMOCOCCUS. J Exp Med. 1931 Apr 30;53(5):625–639. doi: 10.1084/jem.53.5.625. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Heidelberger M., Kendall F. E. SPECIFIC AND NON-SPECIFIC POLYSACCHARIDES OF TYPE IV PNEUMOCOCCUS. J Exp Med. 1931 Apr 30;53(5):625–639. doi: 10.1084/jem.53.5.625. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Heidelberger M., Kendall F. E. STUDIES ON THE PRECIPITIN REACTION : PRECIPITATING HAPTENS; SPECIES DIFFERENCES IN ANTIBODIES. J Exp Med. 1933 Feb 28;57(3):373–379. doi: 10.1084/jem.57.3.373. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Heidelberger M., Kendall F. E. THE PRECIPITIN REACTION BETWEEN TYPE III PNEUMOCOCCUS POLYSACCHARIDE AND HOMOLOGOUS ANTIBODY : III. A QUANTITATIVE STUDY AND A THEORY OF THE REACTION MECHANISM. J Exp Med. 1935 Mar 31;61(4):563–591. doi: 10.1084/jem.61.4.563. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Heidelberger M., Sia R. H., Kendall F. E. SPECIFIC PRECIPITATION AND MOUSE PROTECTION IN TYPE I ANTIPNEUMOCOCCUS SERA. J Exp Med. 1930 Sep 30;52(4):477–483. doi: 10.1084/jem.52.4.477. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tillett W. S., Goebel W. F., Avery O. T. CHEMICAL AND IMMUNOLOGICAL PROPERTIES OF A SPECIES-SPECIFIC CARBOHYDRATE OF PNEUMOCOCCI. J Exp Med. 1930 Nov 30;52(6):895–900. doi: 10.1084/jem.52.6.895. [DOI] [PMC free article] [PubMed] [Google Scholar]