Skip to main content
The Journal of Experimental Medicine logoLink to The Journal of Experimental Medicine
. 1938 Apr 30;67(5):791–797. doi: 10.1084/jem.67.5.791

THE EFFECT OF A TISSUE ENZYME UPON PNEUMOCOCCI

René J Dubos 1, C M MacLeod 1
PMCID: PMC2133632  PMID: 19870755

Abstract

Polymorphonuclear leucocytes contain an enzyme which destroys the basophilic character of beat-killed pneumococci (R and S variants) and inactivates the type specific polysaccharide antigen of encapsulated cells. The same enzyme, however, fails to cause a disintegration of the bacterial cells, or to decompose the capsular polysaccharide itself. The enzyme has been extracted from a number of animal tissues; it appears identical with a purified enzyme extracted from pancreatin and which decomposes yeast nucleic acid. These facts are considered with regard to the failure of rabbits to produce the type specific carbohydrate antibodies when immunized with heat-killed encapsulated pneumococci by the intradermal route.

Full Text

The Full Text of this article is available as a PDF (368.4 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Dubos R. J. THE AUTOLYTIC SYSTEM OF PNEUMOCOCCI. J Exp Med. 1937 May 31;65(6):873–883. doi: 10.1084/jem.65.6.873. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Dubos R. J. THE DECOMPOSITION OF YEAST NUCLEIC ACID BY A HEAT RESISTANT ENZYME. Science. 1937 Jun 4;85(2214):549–550. doi: 10.1126/science.85.2214.549. [DOI] [PubMed] [Google Scholar]
  3. Julianelle L. A. REACTIONS OF RABBITS TO INTRACUTANEOUS INJECTIONS OF PNEUMOCOCCI AND THEIR PRODUCTS : I. THE ANTIBODY RESPONSE. J Exp Med. 1930 Feb 28;51(3):441–448. doi: 10.1084/jem.51.3.441. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from The Journal of Experimental Medicine are provided here courtesy of The Rockefeller University Press

RESOURCES