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. 1938 May 31;67(6):941–952. doi: 10.1084/jem.67.6.941

A METHOD FOR DETERMINING THE DIFFERENTIAL SEDIMENTATION OF PROTEINS IN THE HIGH SPEED CONCENTRATION CENTRIFUGE

Thomas P Hughes 1, Edward G Pickels 1, Frank L Horsfall Jr 1
PMCID: PMC2133646  PMID: 19870767

Abstract

A method is presented for determining the relative degree of sedimentation of proteins and other small particles, either in pure form or in a mixture, utilizing the concentration centrifuge of Bauer and Pickels. Chemical analysis or biological assay of material obtained at different fluid levels by means of a special sampling device is used to measure the degree of sedimentation. The results are reproducible within 10 per cent and, under the conditions of the experiments cited, are characteristic for the proteins thus far investigated.

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Selected References

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  1. Bauer J. H., Pickels E. G. A HIGH SPEED VACUUM CENTRIFUGE SUITABLE FOR THE STUDY OF FILTERABLE VIRUSES. J Exp Med. 1936 Sep 30;64(4):503–528. doi: 10.1084/jem.64.4.503. [DOI] [PMC free article] [PubMed] [Google Scholar]
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