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. 1990 Jul;172(7):3905–3908. doi: 10.1128/jb.172.7.3905-3908.1990

Molecular cloning and characterization of an extracellular protease gene from Aeromonas hydrophila.

O Rivero 1, J Anguita 1, C Paniagua 1, G Naharro 1
PMCID: PMC213372  PMID: 2193924

Abstract

A structural gene which codes for an extracellular protease in Aeromonas hydrophilia SO2/2 and D13 was cloned in Escherichia coli C600-1 by using pBR322 as a vector. The gene codes for a temperature-stable protease with a molecular mass of approximately 38,000 daltons. The protein was secreted to the periplasm of E. coli C600-1 and purified by osmotic shock. Cloned protease (P3) was identical in molecular mass and properties to the one purified from A. hydrophila SO2/2 culture supernatant as an extracellular product.

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Selected References

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