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. 1939 Jan 1;69(1):119–131. doi: 10.1084/jem.69.1.119

AN ELECTROPHORETIC STUDY OF IMMUNE SERA AND PURIFIED ANTIBODY PREPARATIONS

Arne Tiselius 1, Elvin A Kabat 1
PMCID: PMC2133726  PMID: 19870831

Abstract

1. Antibody produced in the horse migrates as a new serum component between the β and γ components, whereas rabbit antibody is electrophoretically identical with the γ globulin component of the serum. 2. In rabbit and monkey antisera the percentage of antibody in the serum and in the γ globulin fraction can be determined by integration of the electrophoresis diagrams of unabsorbed and absorbed sera. Antibody solutions of high purity can be obtained by electrophoretic isolation of the γ globulin of rabbit antisera in which the percentage of antibody to total γ globulin is high. 3. The isoelectric points of pig, cow, horse, and rabbit antibodies have been determined. 4. In horse sera prolonged immunization is accompanied by the formation of another antibody component of lower mobility.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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