Abstract
By adsorbing antigens and antibodies on barium stearate multilayers immunological reactions at surfaces have been studied. Pneumococcus polysaccharide specific antibody systems using purified antibodies from both horse and rabbit sera were investigated. The polysaccharides failed to show visible adsorption, but by alternate treatment with antibody and polysaccharide several layers of antibody could be specifically deposited. With the diphtheria toxin-antitoxin system antitoxin was found to adsorb to layers of toxin but not conversely. The reaction, however, was not specific. Molecular weights calculated from the thickness of adsorbed protein layers, using dissymmetry factors, roughly correspond to molecular weights calculated from sedimentation and diffusion constants.
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Selected References
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- Heidelberger M., Kendall F. E. QUANTITATIVE STUDIES ON ANTIBODY PURIFICATION : I. THE DISSOCIATION OF PRECIPITATES FORMED BY PNEUMOCOCCUS SPECIFIC POLYSACCHARIDES AND HOMOLOGOUS ANTIBODIES. J Exp Med. 1936 Jul 31;64(2):161–172. doi: 10.1084/jem.64.2.161. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Heidelberger M., Kendall F. E., Scherp H. W. THE SPECIFIC POLYSACCHARIDES OF TYPES I, II, AND III PNEUMOCOCCUS : A REVISION OF METHODS AND DATA. J Exp Med. 1936 Sep 30;64(4):559–572. doi: 10.1084/jem.64.4.559. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kabat E. A. THE MOLECULAR WEIGHT OF ANTIBODIES. J Exp Med. 1939 Jan 1;69(1):103–118. doi: 10.1084/jem.69.1.103. [DOI] [PMC free article] [PubMed] [Google Scholar]