Abstract
A non-antigenic mucoid polysaccharide similar to that described by Kendall, Heidelberger, and Dawson was isolated from group C hemolytic streptococci. A simple method for its quantitative estimation is described. By means of this quantitative method, as well as by the direct isolation of the carbohydrates, the size and persistence of capsules in young cultures of various strains have been related to the non-antigenic mucoid polysaccharide. Similarly, invasiveness in different strains has been related to the mucoid polysaccharide. Data indicating the non-haptene nature of this material are presented. An autolytic process, accelerated by bile, is involved in the loss of capsular material from young streptococci.
Full Text
The Full Text of this article is available as a PDF (1,005.9 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Dawson M. H., Olmstead M. THE MUCOID PHASE OF STREPTOCOCCUS HEMOLYTICUS. Science. 1934 Sep 28;80(2074):296–297. doi: 10.1126/science.80.2074.296. [DOI] [PubMed] [Google Scholar]
- Duran-Reynals F. STUDIES ON A CERTAIN SPREADING FACTOR EXISTING IN BACTERIA AND ITS SIGNIFICANCE FOR BACTERIAL INVASIVENESS. J Exp Med. 1933 Jul 31;58(2):161–181. doi: 10.1084/jem.58.2.161. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Elson L. A., Morgan W. T. A colorimetric method for the determination of glucosamine and chondrosamine. Biochem J. 1933;27(6):1824–1828. doi: 10.1042/bj0271824. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Enders J. F., Shaffer M. F., Wu C. J. STUDIES ON NATURAL IMMUNITY TO PNEUMOCOCCUS TYPE III : III. CORRELATION OF THE BEHAVIOR IN VIVO OF PNEUMOCOCCI TYPE III VARYING IN THEIR VIRULENCE FOR RABBITS WITH CERTAIN DIFFERENCES OBSERVED IN VITRO. J Exp Med. 1936 Jul 31;64(2):307–330. doi: 10.1084/jem.64.2.307. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Goebel W. F. THE ISOLATION OF THE BLOOD GROUP A SPECIFIC SUBSTANCE FROM COMMERCIAL PEPTONE. J Exp Med. 1938 Jul 31;68(2):221–227. doi: 10.1084/jem.68.2.221. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hirst G. K., Lancefield R. C. ANTIGENIC PROPERTIES OF THE TYPE-SPECIFIC SUBSTANCE DERIVED FROM GROUP A HEMOLYTIC STREPTOCOCCI. J Exp Med. 1939 Feb 28;69(3):425–445. doi: 10.1084/jem.69.3.425. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Meyer K., Smyth E. M., Dawson M. H. THE NATURE OF THE MUCO-POLYSACCHARIDE OF SYNOVIAL FLUID. Science. 1938 Aug 5;88(2275):129–129. doi: 10.1126/science.88.2275.129. [DOI] [PubMed] [Google Scholar]
- Seastone C. V. Capsules in Young Cultures of Streptococcus hemolyticus. J Bacteriol. 1934 Nov;28(5):481–487. doi: 10.1128/jb.28.5.481-487.1934. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Seastone C. V. HEMOLYTIC STREPTOCOCCUS LYMPHADENITIS IN GUINEA PIGS. J Exp Med. 1939 Sep 30;70(4):347–359. doi: 10.1084/jem.70.4.347. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shaffer M. F., Enders J. F., Wu C. J. STUDIES ON NATURAL IMMUNITY TO PNEUMOCOCCUS TYPE III : II. CERTAIN DISTINGUISHING PROPERTIES OF TWO STRAINS OF PNEUMOCOCCUS TYPE III VARYING IN THEIR VIRULENCE FOR RABBITS, AND THE REAPPEARANCE OF THESE PROPERTIES FOLLOWING R-->S RECONVERSION OF THEIR RESPECTIVE ROUGH DERIVATIVES. J Exp Med. 1936 Jul 31;64(2):281–305. doi: 10.1084/jem.64.2.281. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ward H. K., Lyons C. STUDIES ON THE HEMOLYTIC STREPTOCOCCUS OF HUMAN ORIGIN : I. OBSERVATIONS ON THE VIRULENT, ATTENUATED, AND AVIRULENT VARIANTS. J Exp Med. 1935 Mar 31;61(4):515–530. doi: 10.1084/jem.61.4.515. [DOI] [PMC free article] [PubMed] [Google Scholar]