Abstract
Transposon Tn5 insertions causing anaerobic cysteine auxotrophy were isolated from a Salmonella typhimurium cysI parent (auxotrophic under aerobic but not anaerobic conditions). Insertions in one mutant group appeared to be in cysG. A second group of insertions, designated asr (anaerobic sulfite reduction), were located near map unit 53 on the S. typhimurium chromosome. They did not cause aerobic or anaerobic auxotrophy in a cys1+ background but did prevent dissimilatory sulfite reduction. Plasmids containing asr DNA cloned from wild-type S. typhimurium conferred anaerobic prototrophy and the ability to produce hydrogen sulfide from sulfite on an Escherichia coli cys1 mutant.
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- Barrett E. L., Chang G. W. Cysteine auxotrophs of Salmonella typhimurium which grow without cysteine in a hydrogen/carbon dioxide atmosphere. J Gen Microbiol. 1979 Dec;115(2):513–516. doi: 10.1099/00221287-115-2-513. [DOI] [PubMed] [Google Scholar]
- Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1006/abio.1976.9999. [DOI] [PubMed] [Google Scholar]
- Clark M. A., Barrett E. L. The phs gene and hydrogen sulfide production by Salmonella typhimurium. J Bacteriol. 1987 Jun;169(6):2391–2397. doi: 10.1128/jb.169.6.2391-2397.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cole J. A., Newman B. M., White P. Biochemical and genetic characterization of nirB mutants of Escherichia coli K 12 pleiotropically defective in nitrite and sulphite reduction. J Gen Microbiol. 1980 Oct;120(2):475–483. doi: 10.1099/00221287-120-2-475. [DOI] [PubMed] [Google Scholar]
- Coleman K. J., Cornish-Bowden A., Cole J. A. Purification and properties of nitrite reductase from Escherichia coli K12. Biochem J. 1978 Nov 1;175(2):483–493. doi: 10.1042/bj1750483. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Feinberg A. P., Vogelstein B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983 Jul 1;132(1):6–13. doi: 10.1016/0003-2697(83)90418-9. [DOI] [PubMed] [Google Scholar]
- Hallenbeck P. C., Clark M. A., Barrett E. L. Characterization of anaerobic sulfite reduction by Salmonella typhimurium and purification of the anaerobically induced sulfite reductase. J Bacteriol. 1989 Jun;171(6):3008–3015. doi: 10.1128/jb.171.6.3008-3015.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jeter R. M., Olivera B. M., Roth J. R. Salmonella typhimurium synthesizes cobalamin (vitamin B12) de novo under anaerobic growth conditions. J Bacteriol. 1984 Jul;159(1):206–213. doi: 10.1128/jb.159.1.206-213.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LEVINE M., CURTISS R. Genetic fine structure of the C region and the linkage map of phage P22. Genetics. 1961 Dec;46:1573–1580. doi: 10.1093/genetics/46.12.1573. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LOWE R. H., EVANS H. J. PREPARATION AND SOME PROPERTIES OF A SOLUBLE NITRATE REDUCTASE FROM RHIZOBIUM JAPONICUM. Biochim Biophys Acta. 1964 Jun 1;85:377–389. doi: 10.1016/0926-6569(64)90301-3. [DOI] [PubMed] [Google Scholar]
- Monroe R. S., Kredich N. M. Isolation of Salmonella typhimurium cys genes by transduction with a library of recombinant plasmids packaged in bacteriophage P22HT capsids. J Bacteriol. 1988 Jan;170(1):42–47. doi: 10.1128/jb.170.1.42-47.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sanderson K. E., Roth J. R. Linkage map of Salmonella typhimurium, edition VII. Microbiol Rev. 1988 Dec;52(4):485–532. doi: 10.1128/mr.52.4.485-532.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Siegel L. M., Davis P. S. Reduced nicotinamide adenine dinucleotide phosphate-sulfite reductase of enterobacteria. IV. The Escherichia coli hemoflavoprotein: subunit structure and dissociation into hemoprotein and flavoprotein components. J Biol Chem. 1974 Mar 10;249(5):1587–1598. [PubMed] [Google Scholar]
- VOGEL H. J., BONNER D. M. Acetylornithinase of Escherichia coli: partial purification and some properties. J Biol Chem. 1956 Jan;218(1):97–106. [PubMed] [Google Scholar]