Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1990 Jul;172(7):4115–4117. doi: 10.1128/jb.172.7.4115-4117.1990

Enzymes of phosphoinositide synthesis in secretory vesicles destined for the plasma membrane in Saccharomyces cerevisiae.

A J Kinney 1, G M Carman 1
PMCID: PMC213401  PMID: 2163397

Abstract

CDP-diacylglycerol synthase, phosphatidylinositol synthase, and phosphatidylinositol kinase activities were associated with post-Golgi apparatus secretory vesicles destined for the plasma membrane of Saccharomyces cerevisiae. These results suggest that the plasma membrane is capable of synthesizing both CDP-diacylglycerol and phosphatidylinositol as well as phosphorylating phosphatidylinositol.

Full text

PDF
4115

Images in this article

4116Image
on p.4116

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bae-Lee M. S., Carman G. M. Phosphatidylserine synthesis in Saccharomyces cerevisiae. Purification and characterization of membrane-associated phosphatidylserine synthase. J Biol Chem. 1984 Sep 10;259(17):10857–10862. [PubMed] [Google Scholar]
  2. Becker G. W., Lester R. L. Changes in phospholipids of Saccharomyces cerevisiae associated with inositol-less death. J Biol Chem. 1977 Dec 10;252(23):8684–8691. [PubMed] [Google Scholar]
  3. Belunis C. J., Bae-Lee M., Kelley M. J., Carman G. M. Purification and characterization of phosphatidylinositol kinase from Saccharomyces cerevisiae. J Biol Chem. 1988 Dec 15;263(35):18897–18903. [PubMed] [Google Scholar]
  4. Berridge M. J. Inositol trisphosphate and diacylglycerol: two interacting second messengers. Annu Rev Biochem. 1987;56:159–193. doi: 10.1146/annurev.bi.56.070187.001111. [DOI] [PubMed] [Google Scholar]
  5. Brada D., Schekman R. Coincident localization of secretory and plasma membrane proteins in organelles of the yeast secretory pathway. J Bacteriol. 1988 Jun;170(6):2775–2783. doi: 10.1128/jb.170.6.2775-2783.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1016/0003-2697(76)90527-3. [DOI] [PubMed] [Google Scholar]
  7. Carman G. M., Henry S. A. Phospholipid biosynthesis in yeast. Annu Rev Biochem. 1989;58:635–669. doi: 10.1146/annurev.bi.58.070189.003223. [DOI] [PubMed] [Google Scholar]
  8. Dahl J. S., Dahl C. E. Stimulation of cell proliferation and polyphosphoinositide metabolism in Saccharomyces cerevisiae GL7 by ergosterol. Biochem Biophys Res Commun. 1985 Dec 31;133(3):844–850. doi: 10.1016/0006-291x(85)91211-2. [DOI] [PubMed] [Google Scholar]
  9. Daum G., Heidorn E., Paltauf F. Intracellular transfer of phospholipids in the yeast, Saccharomyces cerevisiae. Biochim Biophys Acta. 1986 Aug 14;878(1):93–101. doi: 10.1016/0005-2760(86)90347-4. [DOI] [PubMed] [Google Scholar]
  10. Fischl A. S., Carman G. M. Phosphatidylinositol biosynthesis in Saccharomyces cerevisiae: purification and properties of microsome-associated phosphatidylinositol synthase. J Bacteriol. 1983 Apr;154(1):304–311. doi: 10.1128/jb.154.1.304-311.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Fischl A. S., Homann M. J., Poole M. A., Carman G. M. Phosphatidylinositol synthase from Saccharomyces cerevisiae. Reconstitution, characterization, and regulation of activity. J Biol Chem. 1986 Mar 5;261(7):3178–3183. [PubMed] [Google Scholar]
  12. Goldstein A., Lampen J. O. Beta-D-fructofuranoside fructohydrolase from yeast. Methods Enzymol. 1975;42:504–511. doi: 10.1016/0076-6879(75)42159-0. [DOI] [PubMed] [Google Scholar]
  13. Henry S. A., Atkinson K. D., Kolat A. I., Culbertson M. R. Growth and metabolism of inositol-starved Saccharomyces cerevisiae. J Bacteriol. 1977 Apr;130(1):472–484. doi: 10.1128/jb.130.1.472-484.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Imai A., Gershengorn M. C. Independent phosphatidylinositol synthesis in pituitary plasma membrane and endoplasmic reticulum. Nature. 1987 Feb 19;325(6106):726–728. doi: 10.1038/325726a0. [DOI] [PubMed] [Google Scholar]
  15. Kaibuchi K., Miyajima A., Arai K., Matsumoto K. Possible involvement of RAS-encoded proteins in glucose-induced inositolphospholipid turnover in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1986 Nov;83(21):8172–8176. doi: 10.1073/pnas.83.21.8172. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Kato H., Uno I., Ishikawa T., Takenawa T. Activation of phosphatidylinositol kinase and phosphatidylinositol-4-phosphate kinase by cAMP in Saccharomyces cerevisiae. J Biol Chem. 1989 Feb 25;264(6):3116–3121. [PubMed] [Google Scholar]
  17. Kelley M. J., Carman G. M. Purification and characterization of CDP-diacylglycerol synthase from Saccharomyces cerevisiae. J Biol Chem. 1987 Oct 25;262(30):14563–14570. [PubMed] [Google Scholar]
  18. Kreibich G., Debey P., Sabatini D. D. Selective release of content from microsomal vesicles without membrane disassembly. I. Permeability changes induced by low detergent concentrations. J Cell Biol. 1973 Aug;58(2):436–462. doi: 10.1083/jcb.58.2.436. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Kuchler K., Daum G., Paltauf F. Subcellular and submitochondrial localization of phospholipid-synthesizing enzymes in Saccharomyces cerevisiae. J Bacteriol. 1986 Mar;165(3):901–910. doi: 10.1128/jb.165.3.901-910.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Nikawa J., Kodaki T., Yamashita S. Primary structure and disruption of the phosphatidylinositol synthase gene of Saccharomyces cerevisiae. J Biol Chem. 1987 Apr 5;262(10):4876–4881. [PubMed] [Google Scholar]
  21. Poole M. A., Homann M. J., Bae-Lee M. S., Carman G. M. Regulation of phosphatidylserine synthase from Saccharomyces cerevisiae by phospholipid precursors. J Bacteriol. 1986 Nov;168(2):668–672. doi: 10.1128/jb.168.2.668-672.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Ramirez R. M., Ishida-Schick T., Krilowicz B. L., Leish B. A., Atkinson K. D. Plasma membrane expansion terminates in Saccharomyces cerevisiae secretion-defective mutants while phospholipid synthesis continues. J Bacteriol. 1983 Jun;154(3):1276–1283. doi: 10.1128/jb.154.3.1276-1283.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Schmidt R., Ackermann R., Kratky Z., Wasserman B., Jacobson B. Fast and efficient purification of yeast plasma membranes using cationic silica microbeads. Biochim Biophys Acta. 1983 Jul 27;732(2):421–427. doi: 10.1016/0005-2736(83)90059-7. [DOI] [PubMed] [Google Scholar]
  24. Uno I., Fukami K., Kato H., Takenawa T., Ishikawa T. Essential role for phosphatidylinositol 4,5-bisphosphate in yeast cell proliferation. Nature. 1988 May 12;333(6169):188–190. doi: 10.1038/333188a0. [DOI] [PubMed] [Google Scholar]
  25. Walworth N. C., Novick P. J. Purification and characterization of constitutive secretory vesicles from yeast. J Cell Biol. 1987 Jul;105(1):163–174. doi: 10.1083/jcb.105.1.163. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES