Abstract
Six hundred and eighty three samples of chicken giblets were examined for salmonellas. Three hundred and forty nine of these were neck and crop specimens and 224 were combined liver and heart samples. Two hundred and ten, in all, contained salmonellas. The technique of examination included pre-enrichment in buffered peptone water at 37 degrees C for 18 h and subculture to three enrichment media: Muller-Kauffmann tetrathionate, selenite F and Rappaport's magnesium chloride malachite green broth. Inocula from buffered peptone water to 10 ml of tetrathionate and selenite were 1 ml in each case. The inoculum from the pre-enrichment medium to 10 ml of Rappaport was 0.005 ml. Tetrathionate and selenite were incubated at 43 degrees C for 48 h. Rappaport's medium was incubated at 37 degrees C for 48 h. Subcultures from all three enrichment broths were made at 24 h and 48 h to brilliant green MacConkey agar. Selective agars were incubated at 37 degrees C for 24 h. The most successful technique for salmonella isolation used Rappaport's medium, which was significantly more efficient than either tetrathionate or selenite. This finding reinforces results obtained using sewage polluted natural water as test material and it is suggested that routine examination of environment samples for salmonellas could be based on Rappaport's medium alone. If S. typhi, S. dublin or subgenus III salmonellas were likely to be present in the sample, the technique described here would require modification.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Chau P. Y., Leung Y. K. Inhibitory action of various plating media on the growth of certain Salmonella serotypes. J Appl Bacteriol. 1978 Dec;45(3):341–345. doi: 10.1111/j.1365-2672.1978.tb04234.x. [DOI] [PubMed] [Google Scholar]
- DIXON J. M. Rapid isolation of salmonellae from faeces. J Clin Pathol. 1961 Jul;14:397–399. doi: 10.1136/jcp.14.4.397. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HARVEY R. W. Choice of a selective medium for the routine isolation of members of the Salmonella group. Mon Bull Minist Health Public Health Lab Serv. 1956 Jun;15:118–124. [PubMed] [Google Scholar]
- HARVEY R. W., PHILLIPS W. P. Survival of Salmonella paratyphi B in sewers; its significance in the investigation of paratyphoid outbreaks. Lancet. 1955 Jul 16;269(6881):137–139. doi: 10.1016/s0140-6736(55)92136-x. [DOI] [PubMed] [Google Scholar]
- Harvey R. W., Price T. H., Hall M. L. Isolations of subgenus 3 salmonellas (arizonas) in Cardiff, 1959-1971. J Hyg (Lond) 1973 Sep;71(3):481–486. doi: 10.1017/s0022172400046465. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Harvey R. W., Price T. H. Isolation of salmonellas from sewage-polluted river water using selenite F and Muller-Kauffmann tetrathionate. J Hyg (Lond) 1976 Dec;77(3):333–339. doi: 10.1017/s0022172400055698. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Harvey R. W., Price T. H., Joynson D. H. Salmonella isolation from hospital areas. J Hyg (Lond) 1979 Dec;83(3):461–468. doi: 10.1017/s0022172400026309. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Harvey R. W., Price T. H., Morgan J. Salmonella surveillance with reference to pigs--Cardiff abattoir, 1968-1975. J Hyg (Lond) 1977 Jun;78(3):439–448. doi: 10.1017/s0022172400056333. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Harvey R. W., Price T. H. Salmonella isolation with Rappaport's medium after pre-enrichment in buffered peptone water using a series of inoculum ratios. J Hyg (Lond) 1980 Aug;85(1):125–128. doi: 10.1017/s0022172400027133. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Harvey R. W., Price T. H. Studies on the isolation of Salmonella dublin. J Hyg (Lond) 1975 Jun;74(3):369–374. doi: 10.1017/s002217240004688x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Harvey R. W., Price T. H., Xirouchaki E. Comparison of selenite F, Muller-Kauffmann tetrathionate and Rappaport's medium for the isolation of salmonellas from sewage-polluted natural water using a pre-enrichment technique. J Hyg (Lond) 1979 Dec;83(3):451–460. doi: 10.1017/s0022172400026292. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KONFORTI N., NAVON B., RAPPAPORT F. A new enrichment medium for certain Salmonellae. J Clin Pathol. 1956 Aug;9(3):261–266. doi: 10.1136/jcp.9.3.261. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Vassiliadis P., Trichopoulos D., Papadakis J., Politi G. Salmonella isolations in abattoirs in Greece. J Hyg (Lond) 1970 Dec;68(4):601–609. doi: 10.1017/s0022172400042534. [DOI] [PMC free article] [PubMed] [Google Scholar]