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. 1982 Jun;88(3):453–461. doi: 10.1017/s0022172400070315

The detection of rubella-specific IgM by an immunosorbent assay with solid-phase attachment of red cells (SPARC).

S A Sexton, J Hodgson, P Morgan-Capner
PMCID: PMC2134101  PMID: 7045216

Abstract

An immunosorbent assay using solid-phase attachment of red cells (SPARC) was used for the detection of rubella-specific IgM. The method is described and the results compared with those obtained by the IgM antibody capture radioimmunoassay (MACRIA). One hundred and ninety-nine sera were investigated for the presence of rubella-specific IgM and only one discrepant result occurred, namely a false positive obtained by MACRIA in a patient with infectious mononucleosis. Rheumatoid factor, heterophile antibody, and rubella-specific IgG did not interfere with the results obtained by the SPARC technique. Advantages of the SPARC technique include the case and lack of expense of testing large numbers of sera, the small volume of sample required and the fact that pretreatment of serum is not necessary.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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