Abstract
The influence of multiple plating of fluid cultures on salmonella isolation from animal feeding stuffs was examined. Four plating were made from broth culture after 24 h at 37 degrees C and four platings from selenite enrichment from 24 h at 43 degrees C. Selenite enrichment followed broth culture which was used as a pre-enrichment stage. Brilliant green MacConkey agar plates were employed for broth subculture and brilliant green MacConkey and desoxycholate citrate agars for selenite subculture. The eight brilliant green plates subcultured from broth and selenite were examined for salmonellas after incubation for 24 h at 37 degrees C. The four desoxycholate citrate agars after 24 h at 37 degrees C were used for motility enrichment. The food sample size was a single 100 g instead of 4 x 25 g cultured in an earlier study. This pooling of samples aimed at technical economy. Quadruple plating played an important part in salmonella isolation from 100 g specimens. The combination of multiple plating with motility enrichment was the most successful technique used.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Chau P. Y., Huang C. T. A one-day selective migration procedure for detecting salmonellae in faeces. J Clin Pathol. 1974 May;27(5):405–407. doi: 10.1136/jcp.27.5.405. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Chau P. Y., Huang C. T. A simple procedure for screening of Salmonellae using a semi-solid enrichment and a semi-solid indicator medium. J Appl Bacteriol. 1976 Oct;41(2):283–294. doi: 10.1111/j.1365-2672.1976.tb00632.x. [DOI] [PubMed] [Google Scholar]
- DIXON J. M. Rapid isolation of salmonellae from faeces. J Clin Pathol. 1961 Jul;14:397–399. doi: 10.1136/jcp.14.4.397. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gabis D. A., Silliker J. H. ICMSF methods studies. II. Comparison of analytical schemes for detection of Salmonella in high-moisture foods. Can J Microbiol. 1974 May;20(5):663–669. doi: 10.1139/m74-102. [DOI] [PubMed] [Google Scholar]
- HARVEY R. W. Choice of a selective medium for the routine isolation of members of the Salmonella group. Mon Bull Minist Health Public Health Lab Serv. 1956 Jun;15:118–124. [PubMed] [Google Scholar]
- HARVEY R. W., THOMSON S. Optimum temperature of incubation for isolation of Salmonellae. Mon Bull Minist Health Public Health Lab Serv. 1953 Jul;12:149–150. [PubMed] [Google Scholar]
- Harvey R. W., Mahabir D. E., Price T. H. A method of secondary enrichment for salmonellas independent of selectively toxic chemicals. J Hyg (Lond) 1966 Sep;64(3):361–366. doi: 10.1017/s002217240004064x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- INO J., GRABER C. D. Recovery of Salmonella from contaminated cultures. U S Armed Forces Med J. 1955 Apr;6(4):586–587. [PubMed] [Google Scholar]
- KROGER E. Zur Bakterienausscheidung bei Typhus-Paratyphus-Keimträgern. Arch Hyg Bakteriol. 1951;135(3):215–221. [PubMed] [Google Scholar]
- Mohit B., Aly R., Bourgeois L. D. A simple single-step immunoimmobilisation method for the detection of Salmonella in the presence of large numbers of other bacteria. J Med Microbiol. 1975 Feb;8(1):173–176. doi: 10.1099/00222615-8-1-173. [DOI] [PubMed] [Google Scholar]
- STUART P. F., PIVNICK H. ISOLATION OF SALMONELLAE BY SELECTIVE MOTILITY SYSTEMS. Appl Microbiol. 1965 May;13:365–372. doi: 10.1128/am.13.3.365-372.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Silliker J. H., Gabis D. A. ICMSF methods studies. I. Comparison of analytical schemes for detection of Salmonella in dried foods. Can J Microbiol. 1973 Apr;19(4):475–479. doi: 10.1139/m73-076. [DOI] [PubMed] [Google Scholar]