Abstract
The serology of 27 heifers found to be positive to culture after inoculation with Brucella abortus strain 544, was studied. Eighteen heifers had previously been vaccinated with strain 19 or strain 45/20 and nine were unvaccinated. Post-infection serum samples were tested for Brucella antibodies by radioimmunoassay (RIA), complement fixation test (CFT), indirect haemolysis test (IHLT) and Rose Bengal plate test (RBPT). All of the unvaccinated heifers showed strong humoral responses to experimental infection in the RIA, CFT, IHLT and RBPT. The CFT and RBPT became positive sooner after infection than the other tests in the unvaccinated heifers. However, in vaccinated heifers the RIA was the most sensitive test early in infection and the results of the RBPT were variable. Three of the vaccinated heifers showed weak and inconsistent humoral responses and, in these animals, the RIA gave fewer false negative reactions than the other tests.
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Selected References
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- Allan G. S., Chappel R. J., Williamson P., McNaught D. J. A quantitative comparison of the sensitivity of serological test for bovine brucellosis to different antibody classes. J Hyg (Lond) 1976 Apr;76(2):287–298. doi: 10.1017/s0022172400055182. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Alton G. G. Recent developments in vaccination against bovine brucellosis. Aust Vet J. 1978 Dec;54(12):551–557. doi: 10.1111/j.1751-0813.1978.tb02410.x. [DOI] [PubMed] [Google Scholar]
- Chappel R. J., Hayes J., Rogerson B. A., Shenfield L. J. The serological response of cattle to vaccines against brucellosis, as measured by the brucellosis radioimmunoassay and other tests. J Hyg (Lond) 1982 Feb;88(1):11–19. doi: 10.1017/s0022172400069849. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Chappel R. J., Williamson P., McNaught D. J., Dalling M. J., Allan G. S. Radioimmunoassay for antibodies against Brucella abortus: a new serological test for bovine brucellosis. J Hyg (Lond) 1976 Dec;77(3):369–376. doi: 10.1017/s0022172400055741. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cordes D. O., Carter M. E. Persistence of Brucella abortus infection in six herds of cattle under brucellosis eradication. N Z Vet J. 1979 Dec;27(12):255–259. doi: 10.1080/00480169.1979.34666. [DOI] [PubMed] [Google Scholar]
- Farrell I. D. The development of a new selective medium for the isolation of Brucella abortus from contaminated sources. Res Vet Sci. 1974 May;16(3):280–286. [PubMed] [Google Scholar]
- Nelson C. J., Anderson R. K., Kimberling C. V., Pietz D. E. Epizootiologic factors of bovine brucellosis: comparative bacteriologic studies of infected herds. Am J Vet Res. 1966 Nov;27(121):1515–1526. [PubMed] [Google Scholar]
- Nicoletti P., Muraschi T. F. Bacteriologic evaluation of serologic test procedures for the diagnosis of brucellosis in problem cattle herds. Am J Vet Res. 1966 May;27(118):689–694. [PubMed] [Google Scholar]
- Plackett P., Cottew G. S., Best S. J. An indirect haemolysis test (IHLT) for bovine brucellosis. Aust Vet J. 1976 Mar;:136–140. doi: 10.1111/j.1751-0813.1976.tb05448.x. [DOI] [PubMed] [Google Scholar]
- Williams M. R., Halliday R. The relatioship between serum immunoglobulin levels and specific antibody production in cows. Res Vet Sci. 1980 Jan;28(1):76–79. [PubMed] [Google Scholar]