Skip to main content
The Journal of Hygiene logoLink to The Journal of Hygiene
. 1983 Feb;90(1):33–39. doi: 10.1017/s0022172400063804

Comparison of standard tube and microagglutination techniques for determining Brucella antibodies.

K A Bettelheim, W J Maskill, J Pearce
PMCID: PMC2134192  PMID: 6401775

Abstract

A microagglutination method for determining the agglutinating and "blocking' antibodies to Brucella abortus is described. A collection of sera from healthy blood donors in two rural areas of New Zealand were tested by the microagglutination methods and the standard methods in tube. The results are compared and show that where discrepancies occur, these are due to the microagglutination methods being more sensitive. It is concluded that these are suitable methods for screening populations.

Full text

PDF
33

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brown S. L., Klein G. C., McKinney F. T., Jones W. L. Safranin O-stained antigen microagglutination test for detection of brucella antibodies. J Clin Microbiol. 1981 Feb;13(2):398–400. doi: 10.1128/jcm.13.2.398-400.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Brown S. L., McKinney F. T., Klein G. C., Jones W. L. Evaluation of a safranin-O-stained antigen microagglutination test for francisella tularensis antibodies. J Clin Microbiol. 1980 Feb;11(2):146–148. doi: 10.1128/jcm.11.2.146-148.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Gilbert G. L., Beaton C. P., Forsyth J. R., Bell C. O. An epidemiological survey of human brucellosis in three Victorian abattoirs. Med J Aust. 1980 May 17;1(10):482–486. doi: 10.5694/j.1326-5377.1980.tb135046.x. [DOI] [PubMed] [Google Scholar]
  4. Metcalfe R. V., Bettelheim K. A., Berry M. E., Hobbs K. M., Thompson A. L., Cole S. P. Studies on antibody levels to Brucella abortus, Toxoplasma gondii and Leptospira serogroups in sera collected by the National Serum Bank during 1974-1976. Zentralbl Bakteriol Orig A. 1979 Dec;245(4):520–526. [PubMed] [Google Scholar]
  5. Metcalfe R. V., Bettelheim K. A., Densham E. B., Pearce J., Sillars H., Thorn C. Studies on antibody levels to Brucella abortus, Toxoplasma gondii and Leptospira serogroups in sera collected in five locations of the North Island of New Zealand. Zentralbl Bakteriol Mikrobiol Hyg A. 1981;249(4):543–556. [PubMed] [Google Scholar]

Articles from The Journal of Hygiene are provided here courtesy of Cambridge University Press

RESOURCES