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. 1982 Oct;89(2):261–267. doi: 10.1017/s0022172400070790

Vi serology in screening of typhoid carriers: improved specificity by detection of Vi antibodies by counterimmunoelectrophoresis.

P Y Chau, R S Tsang
PMCID: PMC2134206  PMID: 7130702

Abstract

A purified soluble Vi antigen was used in counterimmunoelectrophoresis (CIE), passive haemagglutination (HA) and solid phase radio-immunoassay (SPRIA) for detection of serum Vi antibody. Serum Vi antibody was detected in 13 of 14 chronic typhoid carriers by both CIE and HA. SPRIA studies showed that Vi antibodies in sera of these carriers were mainly of the IgG class. Successful treatment with amoxycillin in one carrier, who initially showed a positive Vi test, resulted in a reversion in 6 months to seronegativity as measured by Vi CIE. However, Vi antibody was also detected in 10 sera (3%) from 329 control subjects by HA but not by CIE. When the CIE assay was applied to 1030 serum specimens obtained from hospitalized patients, it was able to detect one typhoid carrier, but one (0.1%) non-carrier also gave a positive Vi CIE test. It is thus concluded that Vi CIE is more specific and no less sensitive than Vi HA for the detection of the typhoid carrier state. The finding by SPRIA that the anti-Vi antibodies present in typhoid carriers' sera were mainly of the IgG class further justified the use of CIE as a precipitation test for their detection.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BAKER E. E., WHITESIDE R. E., BASCH R., DEROW M. A. The VI antigens of the Enterobacteriaceae. I. Purification and chemical properties. J Immunol. 1959 Dec;83:680–696. [PubMed] [Google Scholar]
  2. BOKKENHEUSER V., SMIT P., RICHARDSON N. A CHALLENGE TO THE VALIDITY OF THE VI TEST FOR THE DETECTION OF CHRONIC TYPHOID CARRIERS. Am J Public Health Nations Health. 1964 Sep;54:1507–1513. doi: 10.2105/ajph.54.9.1507. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Barber C., Eylan E. The unfortunate role of precedent in bacteriology. I. The main antigens of salmonellae: the proteins. Zentralbl Bakteriol Orig A. 1976 Jan;234(1):53–59. [PubMed] [Google Scholar]
  4. Chau P. Y., Chan A. C. Modified Vi tests in the screening of typhoid carriers. J Hyg (Lond) 1976 Aug;77(1):97–104. doi: 10.1017/s002217240005556x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Chau P. Y., Forrest C. R. Further study of strontium selenite and selenite F broths for the isolation of Salmonella typhi. J Clin Pathol. 1972 Nov;25(11):966–969. doi: 10.1136/jcp.25.11.966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Chau P. Y., Tsang R. S., Ng A. New method for serological screening of typhoid carriers. Lancet. 1980 Sep 27;2(8196):695–696. doi: 10.1016/s0140-6736(80)92734-8. [DOI] [PubMed] [Google Scholar]
  7. Chernokhvostova E., Luxemburg K. I., Starshinova V., Andreeva N., German G. Study on the production of IgG, IgA- and IgM-antibodies to somatic antigens of Salmonella typhi in humans. Clin Exp Immunol. 1969 Apr;4(4):407–421. [PMC free article] [PubMed] [Google Scholar]
  8. Cooper G. N., Singh J. N. Significance of the V-I haemagglutination test. Med J Aust. 1965 Oct 30;2(18):750–755. [PubMed] [Google Scholar]
  9. Karolcek J., Draskovicová M., Ciznár I. Investigation of the role of Vi antibodies and antibodies of IgA type in the low specific bactericidal activity of the serum in typhoid carriers. Zentralbl Bakteriol Orig A. 1975 Jul;232(2-3):247–251. [PubMed] [Google Scholar]
  10. LANDY M., LAMB E. Estimation of Vi antibody employing erythrocytes treated with purified Vi antigen. Proc Soc Exp Biol Med. 1953 Apr;82(4):593–598. doi: 10.3181/00379727-82-20188. [DOI] [PubMed] [Google Scholar]
  11. Nolan C. M., Feeley J. C., White P. C., Jr, Hambie E. A., Brown S. L., Wong K. H. Evaluation of a new assay for Vi antibody in chronic carriers of Salmonella typhi. J Clin Microbiol. 1980 Jul;12(1):22–26. doi: 10.1128/jcm.12.1.22-26.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Tsang R. S., Chau P. Y., Lam S. K., La Brooy J. T., Rowley D. Antibody response to the lipopolysaccharide and protein antigens of Salmonella typhi during typhoid infection. I. Measurement of serum antibodies by radioimmunoassay. Clin Exp Immunol. 1981 Dec;46(3):508–514. [PMC free article] [PubMed] [Google Scholar]
  13. Tsang R. S., Chau P. Y. Serological diagnosis of typhoid fever by counterimmunoelectrophoresis. Br Med J (Clin Res Ed) 1981 May 9;282(6275):1505–1507. doi: 10.1136/bmj.282.6275.1505. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Wong K. H., Feeley J. C. Isolation of Vi antigen and a simple method for its measurement. Appl Microbiol. 1972 Oct;24(4):628–633. doi: 10.1128/am.24.4.628-633.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]

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