Abstract
Immunoelectrophoresis of ultrasonically disrupted Haemophilus equigenitalis (contagious equine metritis organism) cells against rabbit and equine antisera disclosed at least 11 precipitating antigens. Two of these, a polysaccharide and a lipopolysaccharide-protein complex, were of high molecular weight and located on the cell surface. The remaining antigens were intracellular and were small- to medium-sized proteins. The surface antigens were the most significant in relation to the serological response in infected horses. They also reacted with sera from apparently healthy cattle, but the reason for this was not determined. No serological cross-reaction between H. equigenitalis and species of Achromobacter and Moraxella was detected.
Full text
PDF












Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Benson J. A., Dawson F. L., Durrant D. S., Edwards P. T., Powell D. G. Serological response in mares affected by contagious equine metritis 1977. Vet Rec. 1978 Apr 1;102(13):277–280. doi: 10.1136/vr.102.13.277. [DOI] [PubMed] [Google Scholar]
- Corbel M. J., Brewer R. A. Antibodies to Haemophilus equigenitalis in bovine sera. Vet Rec. 1980 Jan 12;106(2):35–35. doi: 10.1136/vr.106.2.35-a. [DOI] [PubMed] [Google Scholar]
- Corbel M. J. Isolation and partial characterization of a phage receptor from "Brucella neotomae" 5K33. Ann Sclavo. 1977 Jan-Feb;19(1):131–142. [PubMed] [Google Scholar]
- Croxton-Smith P., Benson J. A., Dawson F. L., Powell D. G. A complement fixation test for antibody to the contagious equine metritis organism. Vet Rec. 1978 Sep 23;103(13):275–278. doi: 10.1136/vr.103.13.275. [DOI] [PubMed] [Google Scholar]
- Fernie D. S., Cayzer I., Chalmers S. R. A passive haemagglutination test for the detection of antibodies to the contagious equine metritis organism. Vet Rec. 1979 Mar 24;104(12):260–262. doi: 10.1136/vr.104.12.260. [DOI] [PubMed] [Google Scholar]
- LAURELL C. B. ANTIGEN-ANTIBODY CROSSED ELECTROPHORESIS. Anal Biochem. 1965 Feb;10:358–361. doi: 10.1016/0003-2697(65)90278-2. [DOI] [PubMed] [Google Scholar]
- LEACH A. A., O'SHEA P. C. THE DETERMINATION OF PROTEIN MOLECULAR WEIGHTS OF UP TO 225,000 BY GEL-FILTRATION ON A SINGLE COLUMN OF SEPHADEX G-200 AT 25 DEGREES AND 40 DEGREES. J Chromatogr. 1965 Feb;17:245–251. doi: 10.1016/s0021-9673(00)99864-9. [DOI] [PubMed] [Google Scholar]
- Platt H., Atherton J. G., Simpson D. J. The experimental infection of ponies with contagious equine metritis. Equine Vet J. 1978 Jul;10(3):153–159. doi: 10.1111/j.2042-3306.1978.tb02245.x. [DOI] [PubMed] [Google Scholar]
- Rommel F. A., Dardiri A. H., Sahu S. P., Pierson R. E. Serological identification of the bacterial agent of contagious equine metritis. Vet Rec. 1978 Dec 16;103(25):564–564. doi: 10.1136/vr.103.25.564. [DOI] [PubMed] [Google Scholar]
- SCHEIDEGGER J. J. Une micro-méthode de l'immuno-electrophorèse. Int Arch Allergy Appl Immunol. 1955;7(2):103–110. [PubMed] [Google Scholar]
- Shreeve J. E. Preliminary observations on X-factor growth requirement of the bacterium responsible for CEM. Vet Rec. 1978 Jan 7;102(1):20–20. doi: 10.1136/vr.102.1.20. [DOI] [PubMed] [Google Scholar]
- Smith J. E., Young C. R. Agglutinins to causative organism of contagious equine metritis 1977 in human serum. Lancet. 1978 Jun 10;1(8076):1266–1266. doi: 10.1016/s0140-6736(78)92507-2. [DOI] [PubMed] [Google Scholar]
- Swaney L. M., Breese S. S., Jr Ultrastructure of Haemophilus equigenitalis, causative agent of contagious equine metritis. Am J Vet Res. 1980 Jan;41(1):127–132. [PubMed] [Google Scholar]
- Taylor C. E., Rosenthal R. O. Agglutinins to the causative organism of contagious equine metritis 1977 in human serum. Lancet. 1978 May 13;1(8072):1038–1038. doi: 10.1016/s0140-6736(78)90756-0. [DOI] [PubMed] [Google Scholar]
- Taylor C. E., Rosenthal R. O., Brown D. F., Lapage S. P., Hill L. R., Legros R. M. The causative organism of contagious equine metritis 1977: proposal for a new species to be known as Haemophilus equigenitalis. Equine Vet J. 1978 Jul;10(3):136–144. doi: 10.1111/j.2042-3306.1978.tb02242.x. [DOI] [PubMed] [Google Scholar]
- Timoney P. J., Ward J., Hyde W. A. The application of bioluminescence and gas liquid chromatography for the rapid diagnosis of contagious equine metritis 1977. Vet Rec. 1978 Sep 9;103(11):243–244. doi: 10.1136/vr.103.11.243. [DOI] [PubMed] [Google Scholar]



