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. 1986 Oct;168(1):270–275. doi: 10.1128/jb.168.1.270-275.1986

Biosynthesis of a galactose-and galacturonic acid-containing polysaccharide in Rhizobium meliloti.

R A Ugalde, J A Coira, W J Brill
PMCID: PMC213447  PMID: 3759905

Abstract

Previous work showed that two different strains derived from a culture of Rhizobium meliloti 102F51 differed with respect to phage specificity, agglutinability by alfalfa seed lectin, and synthesis of a galactose-containing polysaccharide (R. A. Ugalde, H. Handelsman, and W. J. Brill, J. Bacteriol. 166:148-154, 1986). Inner membranes from the more competitive strain incorporated galactose from UDP-galactose when a thermostable factor was present. This factor has now been identified as UDP-galacturonic acid. UDP-glucuronic acid was also active as a donor; however, this activity may be due to the presence of a 4-epimerase. Galacturonic acid, together with galactose, is incorporated into the reaction product, which appears to be a polysaccharide formed by several repeating units of these two monosaccharides. Partial acid hydrolysis liberates the disaccharide with galactose at the reducing end.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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