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. 1986 Oct;168(1):440–443. doi: 10.1128/jb.168.1.440-443.1986

Requirement of the Escherichia coli dnaA gene product for plasmid F maintenance.

B C Kline, T Kogoma, J E Tam, M S Shields
PMCID: PMC213474  PMID: 3020005

Abstract

There are DnaA protein-binding sites in at least one F origin of replication, and only potentially leaky dnaA(Ts) mutations had ever been used in previous studies indicating that F replication was independent of the dnaA gene product. Here we show that an Escherichia coli dnaA::Tn10 host which does not make a dnaA gene product cannot sustain autonomous or integrated F plasmid maintenance.

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Selected References

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