Abstract
Seventy-six strains of marine photosynthetic bacteria were analyzed by agarose gel electrophoresis for plasmid DNA content. Among these strains, 12 carried two to four different plasmids with sizes ranging from 3.1 to 11.0 megadaltons. The marine photosynthetic bacterium Rhodopseudomonas sp. NKPB002106 had two plasmids, pRD06S and pRD06L. The smaller plasmid, pRD06S, had a molecular weight of 3.8 megadaltons and was cut at a single site by restriction endonucleases SalI, SmaI, PstI, XhoI, and BglII. Moreover, the marine photosynthetic bacterium Rhodopseudomonas sp. NKPB002106 containing plasmid pRD06 had a satisfactory growth rate (doubling time, 7.5 h), a hydrogen-producing rate of 0.96 mumol/mg (dry weight) of cells per h, and nitrogen fixation capability. Plasmid pRD06S, however, had neither drug resistance nor heavy-metal resistance, and its copy number was less than 10. Therefore, a recombinant plasmid consisting of pRD06S and Escherichia coli cloning vector pUC13 was constructed and cloned in E. coli. The recombinant plasmid was transformed into Rhodopseudomonas sp. NKPB002106. As a result, Rhodopseudomonas sp. NKPB002106 developed ampicillin resistance. Thus, a shuttle vector for gene transfer was constructed for marine photosynthetic bacteria.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Birnboim H. C., Doly J. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 1979 Nov 24;7(6):1513–1523. doi: 10.1093/nar/7.6.1513. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cohen S. N., Chang A. C. Recircularization and autonomous replication of a sheared R-factor DNA segment in Escherichia coli transformants. Proc Natl Acad Sci U S A. 1973 May;70(5):1293–1297. doi: 10.1073/pnas.70.5.1293. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fornari C. S., Watkins M., Kaplan S. Plasmid distribution and analyses in Rhodopseudomonas sphaeroides. Plasmid. 1984 Jan;11(1):39–47. doi: 10.1016/0147-619x(84)90005-2. [DOI] [PubMed] [Google Scholar]
- Gibson K. D., Niederman R. A. Characterization of two circular satellite species of deoxyribonucleic acid in Rhodopseudomonas spheroides. Arch Biochem Biophys. 1970 Dec;141(2):694–704. doi: 10.1016/0003-9861(70)90190-6. [DOI] [PubMed] [Google Scholar]
- Kuhl S. A., Nix D. W., Yoch D. C. Characterization of a Rhodospirillum rubrum plasmid: loss of photosynthetic growth in plasmidless strains. J Bacteriol. 1983 Nov;156(2):737–742. doi: 10.1128/jb.156.2.737-742.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nano F. E., Kaplan S. Plasmid rearrangements in the photosynthetic bacterium Rhodopseudomonas sphaeroides. J Bacteriol. 1984 Jun;158(3):1094–1103. doi: 10.1128/jb.158.3.1094-1103.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rüther U. Construction and properties of a new cloning vehicle, allowing direct screening for recombinant plasmids. Mol Gen Genet. 1980;178(2):475–477. doi: 10.1007/BF00270503. [DOI] [PubMed] [Google Scholar]
- Saunders V. A., Saunders J. R., Bennett P. M. Extrachromosomal deoxyribonucleic acid in wild-type and photosynthetically incompetent strains of Rhodopseudomonas spheroides. J Bacteriol. 1976 Mar;125(3):1180–1187. doi: 10.1128/jb.125.3.1180-1187.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Suyama Y., Gibson J. Satellite DNA in photosynthetic bacteria. Biochem Biophys Res Commun. 1966 Aug 23;24(4):549–553. doi: 10.1016/0006-291x(66)90355-x. [DOI] [PubMed] [Google Scholar]
- Thomas M., Davis R. W. Studies on the cleavage of bacteriophage lambda DNA with EcoRI Restriction endonuclease. J Mol Biol. 1975 Jan 25;91(3):315–328. doi: 10.1016/0022-2836(75)90383-6. [DOI] [PubMed] [Google Scholar]
- van den Hondel C. A., Keegstra W., Borrias W. E., van Arkel G. A. Homology of plasmids in strains of unicellular Cyanobacteria. Plasmid. 1979 Jul;2(3):323–333. doi: 10.1016/0147-619x(79)90016-7. [DOI] [PubMed] [Google Scholar]