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Expression of TGF-α, EGFR, VEGFR2, and HER2 by SW620CE2 cells growing in culture. Western blot and RT-PCR analyses reveal that the SW620CE2 cells do not express (A) EGFR, (B) HER2, or (C) VEGFR2 protein. KM12C human colon cancer cells served as a positive control for EGFR expression, and SKOV3ip1 human ovarian cancer cells served as a positive control for VEGFR2 and HER2 expression. The SW620CE2 cells produced high levels of (D) TGF-α but not (E) EGFR or VEGFR2 mRNA.
